PMID- 12135690
OWN - NLM
STAT- MEDLINE
DCOM- 20030121
LR  - 20211214
IS  - 0009-9120 (Print)
IS  - 0009-9120 (Linking)
VI  - 35
IP  - 4
DP  - 2002 Jun
TI  - Plasma TNF-R1 and insulin concentrations in relation to leptin levels among 
      normal and overweight children.
PG  - 287-92
AB  - OBJECTIVES: Leptin is a multifunctional polypeptide produced primarily by 
      adipocytes and associated with the occurrence of obesity and insulin resistance. 
      Tumor necrosis factor-alpha (TNF-alpha), a polypeptide cytokine produced 
      primarily by mononuclear phagocytes, plays a key role in the initiation of the 
      inflammatory response but has a multitude of effects in many tissues. The 
      interactions between TNF-alpha and insulin on leptin expression are complicated 
      and have not been completely described. The purpose of this study is to evaluate 
      interactions between TNF-alpha and insulin on circulating leptin levels among 
      normal and overweight children. DESIGN AND METHODS: After multi-stage sampling, 
      we randomly selected 1,500 school children in this survey. All children completed 
      a questionnaire on their disease history and lifestyle characteristics. We 
      measured body weight, height, waist and hip circumference of children and 
      calculated BMI and Waist-to-hip ratio (WHR). We also measured plasma tumor 
      necrosis factor-receptor 1 (TNF-R1) by ELISA, plasma insulin and circulating 
      leptin levels by RIA using commercial kits. We divided the children into normal 
      or overweight groups using BMI criteria (85th percentile) to evaluate the 
      relationship of TNF-R1 and insulin on circulating leptin concentrations. RESULTS: 
      In general, boys were taller, heavier and had higher TNF-R1 and lower leptin 
      levels than girls. Plasma leptin levels were positively correlated with 
      anthropometric variables and insulin levels among children in both genders. In 
      multivariate regression analyses, plasma insulin levels were significantly 
      positive associated with leptin levels in normal weight children. Plasma TNF-R1 
      levels were positively associated with leptin levels even after adjusting for BMI 
      in girls only. CONCLUSIONS: From this study, we found that plasma insulin plays 
      certain role in leptin expression among normal weight children. However, TNF-R1 
      plays a more significant role in leptin expression among girls only.
FAU - Chu, Nain Feng
AU  - Chu NF
AD  - Department of Community Medicine, Tri-Service General Hospital, National Defense 
      Medical Center, P.R, Taipei, Taiwan, China. chuepi@ndmctsgh.edu.tw
FAU - Shen, Muh Han
AU  - Shen MH
FAU - Wu, Der Min
AU  - Wu DM
FAU - Shieh, Shyh Ming
AU  - Shieh SM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Antigens, CD)
RN  - 0 (Insulin)
RN  - 0 (LEPR protein, human)
RN  - 0 (Leptin)
RN  - 0 (Receptors, Leptin)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Antigens, CD/*blood
MH  - Body Constitution
MH  - Body Mass Index
MH  - Cross-Sectional Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Insulin/*blood/metabolism
MH  - Leptin/*blood/metabolism
MH  - Male
MH  - Multivariate Analysis
MH  - Obesity/*blood/pathology
MH  - Radioimmunoassay
MH  - Receptors, Leptin
MH  - Receptors, Tumor Necrosis Factor/*blood
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Regression Analysis
MH  - Sex Factors
MH  - Surveys and Questionnaires
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2002/07/24 10:00
MHDA- 2003/01/22 04:00
CRDT- 2002/07/24 10:00
PHST- 2002/07/24 10:00 [pubmed]
PHST- 2003/01/22 04:00 [medline]
PHST- 2002/07/24 10:00 [entrez]
AID - S0009912002003144 [pii]
AID - 10.1016/s0009-9120(02)00314-4 [doi]
PST - ppublish
SO  - Clin Biochem. 2002 Jun;35(4):287-92. doi: 10.1016/s0009-9120(02)00314-4.