PMID- 12138015
OWN - NLM
STAT- MEDLINE
DCOM- 20020819
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 70
IP  - 25
DP  - 2002 May 10
TI  - Somatostatin affects morphology and secretion of pituitary luteinizing hormone 
      (LH) cells in male rats.
PG  - 3019-27
AB  - The somatostatin peptides (SRIH-14, SRIH-28) and their multiple receptors are 
      generally associated with anti-proliferative and anti-secretory actions. This 
      study compared, using standard morphometric measurements and terminal serum LH 
      concentrations, effects of intracerebroventricular (icv) SRIH-14 and SRIH-28 in 
      nanomolar amounts on immunohistochemically identified LH cells in pituitary 
      glands of male rats. Rats received l microg/5 microl of SRIH-14 or SRIH-28 icv on 
      days 1,3, and 5, whereas control rats received only icv saline. Animals were 
      killed 5 days later for serum LH assays. Pituitarys were harvested for PAP 
      immunohistochemistry and morphometry. Morphometric measurements were made by an 
      observer blinded to the treatment group. Histochemically identified LH cells from 
      both SRIH groups appeared smaller, often pycnotic and darkly stained compared to 
      those from saline-treated rats. Both SRIH treatments reduced (p < 0.05) the 
      quantitative morphometric measurements for cell volume, nuclear volume, and 
      relative volume density. Both SRIH treatments also reduced serum LH concentration 
      (p < 0.05), supporting the hypothesis that systemic physiology was altered. 
      Collectively, the data support the opinion that nanomolar amounts of either SRIH 
      peptide, acting on receptors reached from cerebrospinal fluid, exert an 
      anti-secretory effect on LH cells of male rats. Modifications of central SRIH 
      receptors may provide an approach for treatment of male sexual dysfunction and/or 
      be of pathophysiologic significance in these disturbances.
FAU - Starcevic, Vesna
AU  - Starcevic V
AD  - Institute of Physiology, School of Medicine, University of Belgrade, 11001, 
      Belgrade, Yugoslavia. vstar@EUnet.yu
FAU - Milosevic, Verica
AU  - Milosevic V
FAU - Brkic, Branislava
AU  - Brkic B
FAU - Severs, Walter B
AU  - Severs WB
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 51110-01-1 (Somatostatin)
RN  - 75037-27-3 (Somatostatin-28)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Cell Size
MH  - Immunohistochemistry
MH  - Luteinizing Hormone/*metabolism
MH  - Male
MH  - Pituitary Gland/*cytology/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Somatostatin/*pharmacology
MH  - Somatostatin-28
EDAT- 2002/07/26 10:00
MHDA- 2002/08/20 10:01
CRDT- 2002/07/26 10:00
PHST- 2002/07/26 10:00 [pubmed]
PHST- 2002/08/20 10:01 [medline]
PHST- 2002/07/26 10:00 [entrez]
AID - S0024320502015515 [pii]
AID - 10.1016/s0024-3205(02)01551-5 [doi]
PST - ppublish
SO  - Life Sci. 2002 May 10;70(25):3019-27. doi: 10.1016/s0024-3205(02)01551-5.