PMID- 12139743
OWN - NLM
STAT- MEDLINE
DCOM- 20021001
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 118
IP  - 2
DP  - 2002 Aug
TI  - Long-term follow up with conventional cytogenetics and band 13q14 
      interphase/metaphase in situ hybridization monitoring in monoclonal gammopathies 
      of undetermined significance.
PG  - 545-9
AB  - One-third of patients with monoclonal gammopathy of undetermined significance 
      (MGUS) may progress to multiple myeloma (MM) and may develop a long arm deletion 
      of chromosome 13 (13q-). As the incidence of 13q-, time of development and 
      prognostic impact in MGUS patients is still under debate, we decided to perform 
      serial sequential conventional cytogenetics (CC) and metaphase/interphase 
      fluorescence in situ hybridization (FISH) analyses on bone marrow mononuclear 
      cells obtained from 18 asymptomatic, untreated MGUS patients. Median follow up 
      was 30 months (range 6-72). Interphase FISH identified a 13q14 deletion in five 
      out of 18 patients (on clinical diagnosis in one patient and during the follow up 
      in the remaining four patients). Subsequently, metaphase FISH and CC also 
      identified the deletion in four out of five patients. All five of the patients 
      progressed to MM 6-12 months after 13q- identification, without developing any 
      FISH determined JH rearrangements. MM progression also occurred in two other 
      karyotypically normal patients. We conclude that: (i) the extent of the 13q 
      deletion does not vary during the clinical outcome; (ii)13q- plays a crucial role 
      in MGUS/MM pathogenesis and confers a proliferative advantage to clonal plasma 
      cells being initially demonstrated by interphase FISH and only afterwards by 
      metaphase FISH and CC; and (iii) association of 13q- with t(4;14)(p16.3;q32) 
      remains to be demonstrated. However, a transition from MGUS to MM may also occur 
      in patients with normal karyotypes or other abnormalities, suggesting the 
      possibility of distinct pathogenetic pathways.
FAU - Bernasconi, Paolo
AU  - Bernasconi P
AD  - Department of Blood, Heart and Lung Medical Sciences, Division of Haematology, 
      Policlinico San Matteo IRCCS, University of Pavia, 27100-Pavia, Italy. 
      p.bernasconi@smatteo.pv.it
FAU - Cavigliano, Paola Maria
AU  - Cavigliano PM
FAU - Boni, Marina
AU  - Boni M
FAU - Astori, Cesare
AU  - Astori C
FAU - Calatroni, Silvia
AU  - Calatroni S
FAU - Giardini, Ilaria
AU  - Giardini I
FAU - Rocca, Barbara
AU  - Rocca B
FAU - Caresana, Marilena
AU  - Caresana M
FAU - Crosetto, Nicola
AU  - Crosetto N
FAU - Lazzarino, Mario
AU  - Lazzarino M
FAU - Bernasconi, Carlo
AU  - Bernasconi C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 4/genetics
MH  - Disease Progression
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Male
MH  - Metaphase/genetics
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - Paraproteinemias/*genetics
MH  - Prognosis
MH  - Translocation, Genetic
EDAT- 2002/07/26 10:00
MHDA- 2002/10/03 04:00
CRDT- 2002/07/26 10:00
PHST- 2002/07/26 10:00 [pubmed]
PHST- 2002/10/03 04:00 [medline]
PHST- 2002/07/26 10:00 [entrez]
AID - 3651 [pii]
AID - 10.1046/j.1365-2141.2002.03651.x [doi]
PST - ppublish
SO  - Br J Haematol. 2002 Aug;118(2):545-9. doi: 10.1046/j.1365-2141.2002.03651.x.