PMID- 12140176
OWN - NLM
STAT- MEDLINE
DCOM- 20020828
LR  - 20190727
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 182
IP  - 2
DP  - 2002 Jul 15
TI  - Differential regulation of epidermal langerhans cell migration by interleukins 
      (IL)-1alpha and IL-1beta during irritant- and allergen-induced cutaneous immune 
      responses.
PG  - 126-35
AB  - Tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, and IL-18 are 
      all known to contribute to the regulation of epidermal Langerhans cells (LC) 
      migration and the subsequent accumulation of dendritic cells (DC) in draining 
      lymph nodes following skin sensitization. However, the cytokine signals that 
      control these responses following skin irritation have yet to be defined. We 
      demonstrate that IL-1alpha, a cytokine associated with skin injury and 
      inflammation, is able to stimulate the activation and migration from the 
      epidermis of LC and their subsequent accumulation in skin-draining lymph nodes. 
      Stimulation of these responses by IL-1alpha required the local availability of 
      TNF-alpha. Using specific neutralizing antibodies, LC migration induced following 
      skin sensitization with oxazolone (Ox) was found to be dependent upon IL-1beta 
      and independent of a requirement for IL-1alpha. However, the converse was true 
      following stimulation of responses with the nonsensitizing skin irritant sodium 
      lauryl sulfate (SLS). Here, the loss of LC from the epidermis and the 
      accumulation of DC in draining lymph nodes required IL-1alpha and not IL-1beta. 
      Despite utilizing different IL-1 isoforms for LC mobilization, the phenotypic 
      characteristics of DC arriving in draining lymph nodes in response to Ox and SLS 
      were similar with respect to the membrane determinants MHC class II, B7-1, B7-2, 
      and intercellular adhesion molecule-1. These data suggest that contact 
      sensitization and skin irritation employ subtly different cytokine networks in 
      the regulation of LC migration, both involving TNF-alpha but demonstrating 
      differential requirements for IL-1 cytokines. The proposal is that different 
      forms of cutaneous trauma may achieve LC migration through distinct molecular 
      mechanisms.
FAU - Cumberbatch, Marie
AU  - Cumberbatch M
AD  - Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, 
      SK10 4TJ, United Kingdom. Marie.Cumberbatch@Syngenta.com
FAU - Dearman, Rebecca J
AU  - Dearman RJ
FAU - Groves, Richard W
AU  - Groves RW
FAU - Antonopoulos, Christos
AU  - Antonopoulos C
FAU - Kimber, Ian
AU  - Kimber I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Allergens)
RN  - 0 (Interleukin-1)
RN  - 0 (Irritants)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 15646-46-5 (Oxazolone)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Allergens/*pharmacology
MH  - Animals
MH  - Cell Movement/*physiology
MH  - Cell Separation
MH  - Dendritic Cells/drug effects
MH  - Dermatitis, Contact/*immunology
MH  - Epidermal Cells
MH  - Epidermis/drug effects
MH  - Flow Cytometry
MH  - In Vitro Techniques
MH  - Interleukin-1/*pharmacology
MH  - Irritants/*pharmacology
MH  - Langerhans Cells/*physiology
MH  - Lymph Nodes/cytology/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxazolone/pharmacology
MH  - Phenotype
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2002/07/26 10:00
MHDA- 2002/08/29 10:01
CRDT- 2002/07/26 10:00
PHST- 2002/07/26 10:00 [pubmed]
PHST- 2002/08/29 10:01 [medline]
PHST- 2002/07/26 10:00 [entrez]
AID - S0041008X02994421 [pii]
AID - 10.1006/taap.2002.9442 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2002 Jul 15;182(2):126-35. doi: 10.1006/taap.2002.9442.