PMID- 12142611
OWN - NLM
STAT- MEDLINE
DCOM- 20021223
LR  - 20190906
IS  - 0193-1091 (Print)
IS  - 0193-1091 (Linking)
VI  - 24
IP  - 4
DP  - 2002 Aug
TI  - Interferon-gamma treatment induces granulomatous tissue reaction in a case of 
      localized cutaneous leishmaniasis.
PG  - 319-23
AB  - Leishmania spp. suppress macrophage activity as part of their interaction with 
      the immune system. Interferon-gamma (IFNgamma), a cytokine that participates in 
      the activation of macrophages and the killing of intercellular parasites, induces 
      healing of leishmaniasis. We investigated a sequence of local and systemic 
      inflammatory cell parameters after IFNgamma therapy in a patient with chronic, 
      localized, cutaneous leishmaniasis caused by Leishmania donovani. Histology, 
      immunohistochemistry, polymerase chain reaction (PCR) for L. donovani, and 
      analysis of T-cell receptor gene fragments from skin lesions as well as 
      peripheral blood phenotyping were performed before, during, and after IFNgamma 
      therapy. During therapy, epithelioid cell granulomas developed with a high number 
      of lesional human leukocyte antigen (HLA) DR+ macrophages, and HLA-DR expression 
      on monocytes increased to high counts, indicating macrophage activation. 
      Simultaneously, T-cell receptor-beta gene-specific PCR showed a peak at 243 base 
      pairs, indicating clonal expansion of Leishmania-reactive T lymphocytes. After 
      therapy, PCR detected minimal residual leishmanial DNA in healing lesions, 
      suggesting the destruction of the parasites. In conclusion, IFNgamma therapy 
      compensates for the parasite-dependent major histocompatibility complex class II 
      downregulation and induces healing of chronic cutaneous leishmaniasis.
FAU - Haas, Norbert
AU  - Haas N
AD  - Department of Dermatology and Allergy, Charite, Humboldt-University Berlin, 
      Berlin, Germany. norb.haas@charite.de
FAU - Hauptmann, Steffen
AU  - Hauptmann S
FAU - Paralikoudi, Dimitra
AU  - Paralikoudi D
FAU - Muche, Marcus
AU  - Muche M
FAU - Kolde, Gerhard
AU  - Kolde G
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Am J Dermatopathol
JT  - The American Journal of dermatopathology
JID - 7911005
RN  - 0 (DNA, Protozoan)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Animals
MH  - DNA, Protozoan/analysis
MH  - Genes, T-Cell Receptor/genetics
MH  - Granuloma/immunology/pathology/*therapy
MH  - Humans
MH  - Immunophenotyping
MH  - Interferon-gamma/*therapeutic use
MH  - Leishmania donovani/genetics/isolation & purification
MH  - Leishmaniasis, Cutaneous/immunology/pathology/*therapy
MH  - Male
MH  - Receptors, Antigen, T-Cell/analysis
EDAT- 2002/07/27 10:00
MHDA- 2002/12/27 04:00
CRDT- 2002/07/27 10:00
PHST- 2002/07/27 10:00 [pubmed]
PHST- 2002/12/27 04:00 [medline]
PHST- 2002/07/27 10:00 [entrez]
AID - 10.1097/00000372-200208000-00006 [doi]
PST - ppublish
SO  - Am J Dermatopathol. 2002 Aug;24(4):319-23. doi: 10.1097/00000372-200208000-00006.