PMID- 12142916
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 0582-9879 (Print)
IS  - 0582-9879 (Linking)
VI  - 31
IP  - 1
DP  - 1999
TI  - Expression of Brain-derived Neurotrophic Factor (BDNF) Gene in Rat Myoblast 
      Cells.
PG  - 57-60
AB  - Brain-derived neurotrophic factor (BDNF) gene was cloned into retrovirous vector 
      plasmid pLNCX to form the pLNC/BDNF. After packaging in PA317 cell line, the 
      infectious particles were used to infect rat myoblast cell line L-6TG. After 
      selection with G418, the L-6TG/BDNF cells were collected. Results from Southern 
      blot show that bdnf gene has successfully integrated into the L-6TG genomic DNA. 
      The expression of bdnf gene has been proved by Northern blot and Dot blot. The 
      content of BDNF in the culture medium of L-6TG/BDNF was about 25 ng.10(-6) cells 
      per 24 h per ml.
FAU - Liu, Jian
AU  - Liu J
AD  - Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 
      200031, China. sib203@sunm.shcnc.ac.cn
FAU - Jiang, Qiong
AU  - Jiang Q
FAU - Ge, Kai
AU  - Ge K
FAU - Sun, Lan-Ying
AU  - Sun LY
FAU - Liu, Xin-Yuan
AU  - Liu XY
FAU - Zheng, Zhong-Cheng
AU  - Zheng ZC
LA  - eng
PT  - Journal Article
PL  - China
TA  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
JT  - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
JID - 20730160R
EDAT- 2002/07/27 10:00
MHDA- 2002/07/27 10:00
CRDT- 2002/07/27 10:00
PHST- 2002/07/27 10:00 [pubmed]
PHST- 2002/07/27 10:00 [medline]
PHST- 2002/07/27 10:00 [entrez]
PST - ppublish
SO  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(1):57-60.