PMID- 12144533
OWN - NLM
STAT- MEDLINE
DCOM- 20020913
LR  - 20191210
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 68
IP  - 5
DP  - 2002 May
TI  - Comprehensive comparison of FISH, RT-PCR, and RQ-PCR for monitoring the BCR-ABL 
      gene after hematopoietic stem cell transplantation in CML.
PG  - 272-80
AB  - The reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with 
      fluorescence in situ hybridization (FISH) and real-time quantitative RT-PCR 
      (RQ-PCR) for minimal residual disease (MRD) monitoring in 266 post-transplant 
      bone marrow samples from 78 patients with chronic myelogenous leukemia (CML). The 
      sensitivities of FISH to BCR-ABL positive samples determined by first-round (1st) 
      RT-PCR, second-round (2nd) RT-PCR, and RQ-PCR were 64.2%, 25.8%, and 20.7%, 
      respectively. The BCR-ABL/ABL ratio by RQ-PCR had a mean of 0.000 13 in the 1st 
      RT-PCR-negative samples and 1.42 in the 1st RT-PCR-positive samples (P<0.001), 
      and means of 0.000 39 and 0.51 in the 2nd RT-PCR-negative and -positive samples 
      (P< 0.001). The mean ratios of BCR-ABL/ABL by RQ-PCR were significantly different 
      in N/N (1st/2nd RT-PCR) or N/P and P/P (P<0.001), but not in N/N and N/P, which 
      showed that the discriminative power of RQ-PCR is confined to the 1st RT-PCR 
      level. In this respect, monitoring of the 1st RT-PCR might be useful for 
      estimating normalized BCR-ABL levels after transplantation. Nested RT-PCR was of 
      limited use, as RQ-PCR quantified the BCR-ABL transcripts in 60 (91%) of 66 
      samples determined to be negative by 2nd RT-PCR. FISH was significantly 
      correlated with RQ-PCR in FISH-positive samples (n=24, r=0.79, P=0.001). An 
      increase of FISH preceded that of RQ-PCR in a few cases with molecular relapse. 
      By analyzing a large number of samples post-transplant, we found that RQ-PCR 
      might be the most useful assay for MRD monitoring; however, FISH and RT-PCR were 
      found to be useful complementary tools.
FAU - Kim, Yoo-Jin
AU  - Kim YJ
AD  - Catholic Hemopoietic Stem Cell Transplantation Center, Catholic University of 
      Korea, #62 Youido-dong, Youngdeungpo-gu, Seoul, Korea (South), 150-713.
FAU - Kim, Dong-Wook
AU  - Kim DW
FAU - Lee, Seok
AU  - Lee S
FAU - Kim, Hee-Je
AU  - Kim HJ
FAU - Kim, Yoo-Li
AU  - Kim YL
FAU - Hwang, Ji-Yeon
AU  - Hwang JY
FAU - Oh, Il-Hoan
AU  - Oh IH
FAU - Park, Yoon-Hee
AU  - Park YH
FAU - Lee, You-Kyoung
AU  - Lee YK
FAU - Min, Chang-Ki
AU  - Min CK
FAU - Kim, Tai-Gyu
AU  - Kim TG
FAU - Han, Tae-Hee
AU  - Han TH
FAU - Min, Woo-Sung
AU  - Min WS
FAU - Kim, Chun-Choo
AU  - Kim CC
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Bone Marrow/pathology
MH  - Feasibility Studies
MH  - Gene Rearrangement
MH  - Genes, abl/*genetics
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*standards
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/genetics/therapy
MH  - Methods
MH  - Neoplasm, Residual/diagnosis/genetics
MH  - Polymerase Chain Reaction/methods/*standards
MH  - RNA, Messenger/analysis
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction/standards
EDAT- 2002/07/30 10:00
MHDA- 2002/09/14 10:01
CRDT- 2002/07/30 10:00
PHST- 2002/07/30 10:00 [pubmed]
PHST- 2002/09/14 10:01 [medline]
PHST- 2002/07/30 10:00 [entrez]
AID - 00671 [pii]
AID - 10.1034/j.1600-0609.2002.00671.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2002 May;68(5):272-80. doi: 10.1034/j.1600-0609.2002.00671.x.