PMID- 12144534
OWN - NLM
STAT- MEDLINE
DCOM- 20020913
LR  - 20191210
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 68
IP  - 5
DP  - 2002 May
TI  - Enrichment of normal progenitors in counter-flow centrifugal elutriation (CCE) 
      fractions of fresh chronic myeloid leukemia leukapheresis products.
PG  - 281-8
AB  - OBJECTIVES: The aim of this study was to assess the suitability of a technique 
      based on counter-flow centrifugal elutriation (CCE), which should allow one to 
      enrich chronic myeloid leukemia (CML) patients' unstimulated native leukapheresis 
      product (nLP) in CD34+ HLADR- cells and BCR-ABL negative cells. METHODS: Six 
      newly diagnosed CML patients were subjected to leukapheresis, and the products 
      were subfractionated with the use of CCE. nLP and all fractions were studied for 
      the presence of CD34+ cells and a proportion of BCR-ABL fluorescence in situ 
      hybridization (FISH)+ cells. RESULTS: CCE fractions with a high flow rate 
      contained the highest proportion of CD34+ cells [mean (SEM) 6.89% (3.88)]. 
      However, CD34+ cells present in low-rate CCE fractions showed a higher proportion 
      of HLADR-[49.6% (13.5 in 70 mL min-1) and 21.5% (11.6 in 110 mL min-1)] than 
      those in 170 mL min-1[3.2% (2.5)] and "rotor off" [3.4% (1.9)]. This was 
      associated with lower proportions of BCR-ABL FISH+[8.1% (4.8) and 1.9 (1.7)] and 
      smaller BCR-ABL to ABL transcript ratios [0.58 (17) and 0.26 (0.08) in 70 and 110 
      mL min-1] fractions as compared to 140 and 170 mL min-1 fractions [21.6% (5.2) 
      and 31.6% (15.3) for BCR-ABL FISH+ cells and 0.75 (0.16) and 0.90 (0.24) for 
      BCR-ABL/ABL]. Fractions with the lowest proportions of BCR-ABL-positive cells and 
      the lowest BCR-ABL/ABL transcript ratios (110 mL min-1) contained from 1.3 x 106 
      to 82.7 x 106 (median: 3.97 x 106) CD34+ cells. CONCLUSIONS: In the present study 
      we have shown that CCE may be used effectively to obtain nLP fractions enriched 
      in normal hematopoietic progenitors.
FAU - Dlubek, Dorota
AU  - Dlubek D
AD  - Lower Silesian Center for Cellular Transplantation, Institute of Immunology and 
      Experimental Therapy, Polish Academy of Science, Rudolf Weigl 12, 53-114 Wroclaw, 
      Poland.
FAU - Dybko, Jaroslaw
AU  - Dybko J
FAU - Wysoczanska, Barbara
AU  - Wysoczanska B
FAU - Laba, Anna
AU  - Laba A
FAU - Klimczak, Aleksandra
AU  - Klimczak A
FAU - Kryczek, Ilona
AU  - Kryczek I
FAU - Konopka, Lech
AU  - Konopka L
FAU - Lange, Andrzej
AU  - Lange A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Antigens, CD34)
RN  - 0 (HLA-DR Antigens)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/analysis
MH  - Cell Separation/methods
MH  - Centrifugation/methods
MH  - Feasibility Studies
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Genes, abl
MH  - HLA-DR Antigens/analysis
MH  - *Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Leukapheresis/*methods/standards
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood/*therapy
MH  - Male
MH  - Middle Aged
MH  - Transplantation, Autologous/methods
EDAT- 2002/07/30 10:00
MHDA- 2002/09/14 10:01
CRDT- 2002/07/30 10:00
PHST- 2002/07/30 10:00 [pubmed]
PHST- 2002/09/14 10:01 [medline]
PHST- 2002/07/30 10:00 [entrez]
AID - 1o682 [pii]
AID - 10.1034/j.1600-0609.2002.01682.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2002 May;68(5):281-8. doi: 10.1034/j.1600-0609.2002.01682.x.