PMID- 12146869
OWN - NLM
STAT- MEDLINE
DCOM- 20030102
LR  - 20190922
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 36
IP  - 11
DP  - 2002 Jun
TI  - Successive changes in community structure of an ethylbenzene-degrading 
      sulfate-reducing consortium.
PG  - 2813-23
AB  - The microbial community structure and successive changes in a mesophilic 
      ethylbenzene-degrading sulfate-reducing consortium were for the first time 
      clarified by the denaturing gradient gel electrophoresis (DGGE) analysis of the 
      PCR amplified 16S rRNA gene fragments. At least ten bands on the DGGE gel were 
      detected in the stationary phase. Phylogenetic analysis of the DGGE bands 
      revealed that the consortium consisted of different eubacterial phyla including 
      the delta subgroup of Proteobacteria, the order Sphingobacteriales, the order 
      Spirochaetales, and the unknown bacterium. The most abundant band C was closely 
      related to strain mXyS1, an m-xylene-degrading sulfate-reducing bacterium (SRB), 
      and occurred as a sole band on DGGE gels in the logarithmic growth phase that 40% 
      ethylbenzene was consumed accompanied by sulfide production. During further 
      prolonged incubation, the dominancy of band C did not change. These results 
      suggest that SRB corresponds to the most abundant band C and contributes mainly 
      to the degradation of ethylbenzene coupled with sulfate reduction.
FAU - Nakagawa, Tatsunori
AU  - Nakagawa T
AD  - Department of Biological Science, Graduate School of Science, Tokyo Metropolitan 
      University, Japan.
FAU - Sato, Shinya
AU  - Sato S
FAU - Yamamoto, Yoko
AU  - Yamamoto Y
FAU - Fukui, Manabu
AU  - Fukui M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Benzene Derivatives)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Water Pollutants)
RN  - L5I45M5G0O (ethylbenzene)
SB  - IM
MH  - Benzene Derivatives/*metabolism
MH  - Biodegradation, Environmental
MH  - DNA, Bacterial/*analysis
MH  - Polymerase Chain Reaction
MH  - Population Dynamics
MH  - Sulfur-Reducing Bacteria/*genetics
MH  - *Water Microbiology
MH  - Water Pollutants/*metabolism
EDAT- 2002/07/31 10:00
MHDA- 2003/01/03 04:00
CRDT- 2002/07/31 10:00
PHST- 2002/07/31 10:00 [pubmed]
PHST- 2003/01/03 04:00 [medline]
PHST- 2002/07/31 10:00 [entrez]
AID - S0043-1354(01)00502-4 [pii]
AID - 10.1016/s0043-1354(01)00502-4 [doi]
PST - ppublish
SO  - Water Res. 2002 Jun;36(11):2813-23. doi: 10.1016/s0043-1354(01)00502-4.