PMID- 12146891
OWN - NLM
STAT- MEDLINE
DCOM- 20020911
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 66
IP  - 3
DP  - 2002 Jul
TI  - Mechanical transmission of porcine reproductive and respiratory syndrome virus by 
      mosquitoes, Aedes vexans (Meigen).
PG  - 191-5
AB  - The objective of this study was to determine whether porcine reproductive and 
      respiratory syndrome virus (PRRSV) could be transmitted to naive pigs by 
      mosquitoes following feeding on infected pigs. During each of 4 replicates, 
      mosquito-to-pig contact took place on days 5, 6, and 7 after PRRSV infection of 
      the donor pig. A total of 300 mosquitoes [Aedes vexans (Meigen)] were allowed to 
      feed on each viremic donor pig, housed in an isolation room. After 30 to 60 s, 
      feeding was interrupted, and the mosquitoes were manually transferred in small 
      plastic vials and allowed to feed to repletion on a naive recipient pig housed in 
      another isolation room. Prior to contact with the recipient pig, the mosquitoes 
      were transferred to clean vials. Swabs were collected from the exterior surface 
      of all vials, pooled, and tested for PRRSV. Separate personnel handled the donor 
      pig, the recipient pig, and the vial-transfer procedure. Transmission of PRRSV 
      from the donor to the recipient pig occurred in 2 out of 4 replicates. The PRRSV 
      isolated from the infected recipient pigs was nucleic-acid-sequenced and found to 
      be 100% homologous with the virus used to infect the donor pigs. Homogenates of 
      mosquito tissues collected in all replicates were positive by either polymerase 
      chain reaction or swine bioassay. All control pigs remained PRRSV negative, and 
      PRRSV was not detected on the surface of the vials. This study indicates that 
      mosquitoes (A. vexans) can serve as mechanical vectors of PRRSV.
FAU - Otake, Satoshi
AU  - Otake S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, St. Paul 55108, USA.
FAU - Dee, Scott A
AU  - Dee SA
FAU - Rossow, Kurt D
AU  - Rossow KD
FAU - Moon, Roger D
AU  - Moon RD
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (DNA, Viral)
SB  - IM
EIN - Can J Vet Res 2002 Oct;66(4):294
MH  - Aedes/*virology
MH  - Animals
MH  - DNA, Viral/blood
MH  - Insect Vectors/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*growth & 
      development
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Viremia/veterinary
PMC - PMC227003
EDAT- 2002/07/31 10:00
MHDA- 2002/09/12 10:01
PMCR- 2002/10/01
CRDT- 2002/07/31 10:00
PHST- 2002/07/31 10:00 [pubmed]
PHST- 2002/09/12 10:01 [medline]
PHST- 2002/07/31 10:00 [entrez]
PHST- 2002/10/01 00:00 [pmc-release]
AID - 0000000-200207000-00008 [pii]
PST - ppublish
SO  - Can J Vet Res. 2002 Jul;66(3):191-5.