PMID- 12147307 OWN - NLM STAT- MEDLINE DCOM- 20021028 LR - 20190701 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 328 IP - 3 DP - 2002 Aug 16 TI - The novel catecholaminergic and serotoninergic activity enhancer R-(-)-1-(benzofuran-2-yl)-2-propylaminopentane up-regulates neurotrophic factor synthesis in mouse astrocytes. PG - 205-8 AB - We investigated the effects of the novel catecholaminergic and serotoninergic activity enhancer R-(-)-1-(benzofuran-2-yl)-2-propylaminopentane ((-)-BPAP) on the synthesis and secretion of neurotrophins, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neurotrophic factor (GDNF), in cultured mouse astrocytes. The protein and mRNA levels of the neurotrophic factors were measured using the enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction methods, respectively. The amounts of NGF, BDNF, and GDNF secreted from astrocytes into the culture medium increased by up to 120, two, and seven times higher than those of the control, respectively, by treatment with 0.35 mM (-)-BPAP for 24 h. The increases in NGF and GDNF induced by the treatment with (-)-BPAP was inhibited by concomitant treatment with actinomycin D for transcriptional blockade. Furthermore, the treatment with (-)-BPAP for 6 h increased the mRNA expression of NGF, BDNF, and GDNF. These results suggest that (-)-BPAP up-regulated neurotrophic factor synthesis in cultured astrocytes. FAU - Ohta, Kiyoe AU - Ohta K AD - Clinical Research Center, Utano National Hospital, Narutaki, Ukyo, 616-8255, Kyoto, Japan. kiyoeohta@mail.goo.ne.jp FAU - Ohta, Mitsuhiro AU - Ohta M FAU - Mizuta, Ikuko AU - Mizuta I FAU - Fujinami, Aya AU - Fujinami A FAU - Shimazu, Seiichiro AU - Shimazu S FAU - Sato, Noriyuki AU - Sato N FAU - Yoneda, Fumio AU - Yoneda F FAU - Hayashi, Kyozo AU - Hayashi K FAU - Kuno, Sadako AU - Kuno S LA - eng PT - Journal Article PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (1-(benzofuran-2-yl)-2-propylaminopentane) RN - 0 (Benzofurans) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Catecholamines) RN - 0 (Gdnf protein, mouse) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (RNA, Messenger) RN - 333DO1RDJY (Serotonin) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Animals MH - Astrocytes/*drug effects/*metabolism MH - Benzofurans/*pharmacology MH - Brain-Derived Neurotrophic Factor/genetics/metabolism MH - Catecholamines/physiology MH - Cells, Cultured MH - Enzyme-Linked Immunosorbent Assay MH - Glial Cell Line-Derived Neurotrophic Factor MH - Mice MH - Mice, Inbred ICR MH - Nerve Growth Factor/genetics/metabolism MH - Nerve Growth Factors/*biosynthesis/genetics/metabolism MH - Nerve Tissue Proteins/genetics/metabolism MH - RNA, Messenger/metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - Serotonin/physiology MH - Up-Regulation EDAT- 2002/07/31 10:00 MHDA- 2002/10/29 04:00 CRDT- 2002/07/31 10:00 PHST- 2002/07/31 10:00 [pubmed] PHST- 2002/10/29 04:00 [medline] PHST- 2002/07/31 10:00 [entrez] AID - S0304394002004615 [pii] AID - 10.1016/s0304-3940(02)00461-5 [doi] PST - ppublish SO - Neurosci Lett. 2002 Aug 16;328(3):205-8. doi: 10.1016/s0304-3940(02)00461-5.