PMID- 12147325
OWN - NLM
STAT- MEDLINE
DCOM- 20021028
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 328
IP  - 3
DP  - 2002 Aug 16
TI  - Excitotoxic injury induces production of monocyte chemoattractant protein-1 in 
      rat cortico-striatal slice cultures.
PG  - 277-80
AB  - The effect of excitotoxic injury on the production of monocyte chemoattractant 
      protein-1 (MCP-1) was examined in rat cortico-striatal slice cultures. Treatment 
      with 50 microM N-methyl-D-aspartate (NMDA) for 4 h, which caused severe damage in 
      neurons, induced the production of MCP-1 in astrocytes. Production levels were 
      markedly elevated immediately after the treatment, peaked at 4-8 h, and had 
      decreased to nearly the basal level by 72 h. Since the treatment promoted the 
      release of MCP-1 in the slice cultures, but not in the enriched astrocyte 
      cultures, it is unlikely that NMDA directly acted on the astrocytes. These 
      results suggest that information on neuronal injury induced by NMDA is 
      transmitted to astrocytes to induce the production of MCP-1. Organotypic slice 
      cultures are useful for investigating the inflammatory responses of astrocytes in 
      the process of neuronal injury.
FAU - Katayama, Takahiro
AU  - Katayama T
AD  - Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, 
      Kyoto University, 606-8501, Kyoto, Japan.
FAU - Minami, Masabumi
AU  - Minami M
FAU - Nakamura, Mika
AU  - Nakamura M
FAU - Ito, Misato
AU  - Ito M
FAU - Katsuki, Hiroshi
AU  - Katsuki H
FAU - Akaike, Akinori
AU  - Akaike A
FAU - Satoh, Masamichi
AU  - Satoh M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Chemokine CCL2)
RN  - 0 (Neurotoxins)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/*drug effects/*metabolism/pathology
MH  - Chemokine CCL2/*biosynthesis
MH  - Corpus Striatum/*drug effects/*metabolism/pathology
MH  - In Vitro Techniques
MH  - Neurons/pathology
MH  - Neurotoxins/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
EDAT- 2002/07/31 10:00
MHDA- 2002/10/29 04:00
CRDT- 2002/07/31 10:00
PHST- 2002/07/31 10:00 [pubmed]
PHST- 2002/10/29 04:00 [medline]
PHST- 2002/07/31 10:00 [entrez]
AID - S0304394002005505 [pii]
AID - 10.1016/s0304-3940(02)00550-5 [doi]
PST - ppublish
SO  - Neurosci Lett. 2002 Aug 16;328(3):277-80. doi: 10.1016/s0304-3940(02)00550-5.