PMID- 12149213
OWN - NLM
STAT- MEDLINE
DCOM- 20020912
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 100
IP  - 4
DP  - 2002 Aug 15
TI  - Functional interleukin-7 receptors (IL-7Rs) are expressed by marrow stromal 
      cells: binding of IL-7 increases levels of IL-6 mRNA and secreted protein.
PG  - 1318-25
AB  - DNA spotted microarrays were used to compare gene expression profiles from 2 
      functionally distinct human marrow stromal cell lines: HS-27a, which supports 
      cobblestone area formation by early hematopoietic progenitors, and HS-5, which 
      secretes multiple cytokines that support the proliferation of committed 
      progenitors. One unexpected result was the high level of interleukin-7 receptor 
      (IL-7R) gene expression in HS-27a stromal cells. Northern blot analysis confirmed 
      the IL-7R RNA expression, and Western blots for the IL-7R protein detected both a 
      full-length (90-kd) IL-7R and a smaller 30-kd fragment in both HS-27a cells and 
      primary stromal cell cultures, whereas only the 90-kd receptor protein was 
      detected in peripheral blood mononuclear cells. Biotinylated IL-7 was shown to 
      bind to HS-27a cells under physiologic conditions, and this binding was inhibited 
      by blocking anti-IL-7 antibodies. Tyrosine phosphorylation of several proteins 
      (55 kd, 30 kd, and 24 kd) in HS-27a cells was rapidly increased after incubation 
      with recombinant IL-7. One of the phosphorylated proteins proved to be the 30-kd 
      IL-7R fragment. Exposure of HS-27a cells to IL-7 resulted in a 10-fold increase 
      in secretion of IL-6 into culture supernatants but no increase in the cytokines 
      stromal cell-derived factor 1, macrophage inflammatory protein 1 alpha, or IL-1 
      beta. The up-regulation of IL-6 secretion is associated with a rapid but 
      transient increase in detectable levels of IL-6 messenger RNA. These data suggest 
      that IL-7 may function to regulate the milieu of the microenvironment by 
      modulating IL-6 secretion by the IL-7R-expressing stromal elements.
FAU - Iwata, Mineo
AU  - Iwata M
AD  - Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 
      98109, USA. miwata@fhcrc.org
FAU - Graf, Lynn
AU  - Graf L
FAU - Awaya, Norihiro
AU  - Awaya N
FAU - Torok-Storb, Beverly
AU  - Torok-Storb B
LA  - eng
GR  - CA15704/CA/NCI NIH HHS/United States
GR  - DK56465/DK/NIDDK NIH HHS/United States
GR  - HL62923/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-7)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Recombinant Proteins)
RN  - 21820-51-9 (Phosphotyrosine)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Bone Marrow Cells/*metabolism
MH  - Cell Division
MH  - Cell Line
MH  - Cells, Cultured
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Gene Expression
MH  - Humans
MH  - Interleukin-6/genetics/*metabolism
MH  - Interleukin-7/immunology/*metabolism/pharmacology
MH  - Phosphorylation
MH  - Phosphotyrosine/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Interleukin-7/*genetics/metabolism
MH  - Recombinant Proteins/metabolism/pharmacology
MH  - Stromal Cells/*metabolism
EDAT- 2002/08/01 10:00
MHDA- 2002/09/13 10:01
CRDT- 2002/08/01 10:00
PHST- 2002/08/01 10:00 [pubmed]
PHST- 2002/09/13 10:01 [medline]
PHST- 2002/08/01 10:00 [entrez]
AID - S0006-4971(20)59324-5 [pii]
AID - 10.1182/blood-2002-01-0062 [doi]
PST - ppublish
SO  - Blood. 2002 Aug 15;100(4):1318-25. doi: 10.1182/blood-2002-01-0062.