PMID- 12149226
OWN - NLM
STAT- MEDLINE
DCOM- 20020912
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 100
IP  - 4
DP  - 2002 Aug 15
TI  - Genomic abnormalities in monoclonal gammopathy of undetermined significance.
PG  - 1417-24
AB  - Translocations involving immunoglobulin (Ig) loci and chromosome 13 monosomy 
      (Delta 13) are frequent cytogenetic findings in multiple myeloma (MM). Similar 
      chromosomal aberrations have been identified in the monoclonal gammopathy of 
      undetermined significance (MGUS), but their prevalence and significance remain 
      uncertain. Bone marrow from 72 patients with MGUS (n = 62) and smoldering MM (n = 
      10) was evaluated for translocations between the Ig heavy chain (IgH) and 
      chromosomes 4, 11, and 16, translocations involving Ig light chain-lambda 
      (IgL-lambda, and Delta 13. Fluorescence in situ hybridization (FISH) analysis was 
      done on clonal plasma cells (PCs) detected by immunofluorescence (cIg-FISH) of 
      the cytoplasmic light chain. We also studied cells for cyclin D1 and FGFR3 
      up-regulation by immunohistochemistry and immunofluorescence, respectively. 
      Twenty-seven (46%) of 59 patients had IgH translocations, and 4 (11%) of 37 had 
      an IgL-lambda translocation. A t(11;14)(q13;q32) was found in 15 (25%) of 59 
      patients, a t(4;14)(p16.3;q32) in 9% of patients, and a t(14;16)(q32;q23) in 5% 
      of patients. All patients with t(4;14)(p16.3;q32) tested (n = 3) had intense 
      cytoplasmic fluorescence with an anti-FGFR3 antibody. PC nuclear staining of 
      cyclin D1 was only observed in patients with t(11;14)(q13;q32); Delta 13 was 
      detected in the clonal PCs in 50% of patients. The percentage of abnormal PCs 
      varied with any given abnormality. No obvious clinical or biologic correlations 
      were associated with these chromosome abnormalities. Similar translocations are 
      found in both MGUS and MM, including t(4;14)(p16.3;q32) and t(14;16)(q32;q23). 
      Moreover, Delta 13 is common in MGUS and unlikely to play a predominant role in 
      the evolution of MGUS to MM.
FAU - Fonseca, Rafael
AU  - Fonseca R
AD  - Division of Hematology and Department of Internal Medicine, Mayo Clinic, 
      Rochester, MN 55905, USA. fonesca.rafael@mayo.edu
FAU - Bailey, Richard J
AU  - Bailey RJ
FAU - Ahmann, Gregory J
AU  - Ahmann GJ
FAU - Rajkumar, S Vincent
AU  - Rajkumar SV
FAU - Hoyer, James D
AU  - Hoyer JD
FAU - Lust, John A
AU  - Lust JA
FAU - Kyle, Robert A
AU  - Kyle RA
FAU - Gertz, Morie A
AU  - Gertz MA
FAU - Greipp, Philip R
AU  - Greipp PR
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
GR  - P01 CA62242/CA/NCI NIH HHS/United States
GR  - R01 CA83724-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.10.1 (FGFR3 protein, human)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 3)
SB  - IM
CIN - Blood. 2003 Feb 15;101(4):1653. PMID: 12560243
MH  - Aneuploidy
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 14
MH  - Chromosomes, Human, Pair 16
MH  - Chromosomes, Human, Pair 4
MH  - Cyclin D1/analysis
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin Light Chains/genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Multiple Myeloma/genetics
MH  - Paraproteinemias/*genetics
MH  - Prognosis
MH  - *Protein-Tyrosine Kinases
MH  - Receptor, Fibroblast Growth Factor, Type 3
MH  - Receptors, Fibroblast Growth Factor/analysis
MH  - Translocation, Genetic
EDAT- 2002/08/01 10:00
MHDA- 2002/09/13 10:01
CRDT- 2002/08/01 10:00
PHST- 2002/08/01 10:00 [pubmed]
PHST- 2002/09/13 10:01 [medline]
PHST- 2002/08/01 10:00 [entrez]
AID - S0006-4971(20)59337-3 [pii]
PST - ppublish
SO  - Blood. 2002 Aug 15;100(4):1417-24.