PMID- 12149411
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 8
IP  - 8
DP  - 2002 Aug
TI  - Detection of HLA-G by a specific sandwich ELISA using monoclonal antibodies G233 
      and 56B.
PG  - 776-84
AB  - Human leukocyte antigen (HLA)-G, which is mainly expressed at the maternal-fetal 
      interface, may play a role in the immune tolerance of the semi-allogenic fetus by 
      the mother. Functional studies have shown that HLA-G is indeed a potential 
      modulator of different immune responses. Therefore, it is of interest to study 
      the level of expression of soluble HLA-G in several biological fluids derived 
      from women with and without fertility problems. In order to measure soluble 
      HLA-G, a reliable and sensitive HLA-G specific sandwich ELISA is required. Here, 
      we describe such an ELISA in which G233 is used as the coating antibody and 56B 
      as the detecting antibody. In comparison with two other assays, this assay shows 
      highest responses to recombinant HLA-G and native HLA-G in primary trophoblast 
      culture supernatant and high responses to HLA-G in amniotic fluid. No HLA-G in 
      follicular fluid or preimplantation embryo culture supernatant could be detected.
FAU - Van Lierop, M J C
AU  - Van Lierop MJ
AD  - Department of Pharmacology, NV Organon, 5342 CC Oss, University of Cambridge, UK. 
      marie.jose.vanlierop@organon.com
FAU - Wijnands, F
AU  - Wijnands F
FAU - Loke, Y W
AU  - Loke YW
FAU - Emmer, P M
AU  - Emmer PM
FAU - Lukassen, H G M
AU  - Lukassen HG
FAU - Braat, D D M
AU  - Braat DD
FAU - van der Meer, A
AU  - van der Meer A
FAU - Mosselman, S
AU  - Mosselman S
FAU - Joosten, I
AU  - Joosten I
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Amniotic Fluid/metabolism
MH  - Antibodies, Monoclonal
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Follicular Fluid/metabolism
MH  - HLA Antigens/*analysis
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*analysis
MH  - Humans
EDAT- 2002/08/01 10:00
MHDA- 2003/02/14 04:00
CRDT- 2002/08/01 10:00
PHST- 2002/08/01 10:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/08/01 10:00 [entrez]
AID - 10.1093/molehr/8.8.776 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2002 Aug;8(8):776-84. doi: 10.1093/molehr/8.8.776.