PMID- 12151738
OWN - NLM
STAT- MEDLINE
DCOM- 20020816
LR  - 20231213
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 74
IP  - 2
DP  - 2002 Jul 27
TI  - Differentiation and functional maturation of human CD14(+) adherent peripheral 
      blood monocytes by xenogeneic endothelial cells: up-regulation of costimulation, 
      cytokine generation, and toll-like receptors.
PG  - 243-52
AB  - BACKGROUND: Dendritic cells (DCs) can be generated from peripheral blood 
      mononuclear cells (PBMC) after stimulation with exogenous granulocyte-macrophage 
      colony stimulating factor and interleukin (IL)-4. Further, extravasation of 
      monocytes through human endothelial cells can also cause differentiation, 
      maturation, and expression of DC-specific phenotype. However, it is unclear 
      whether human DCs can be generated from monocytes under the influence of 
      xenogeneic endothelial cells in the absence of exogenous cytokines. We therefore 
      analyzed and compared the effect of human and porcine endothelial cells on the 
      differentiation of human monocytes into DC. METHODS: Adherent peripheral blood 
      CD14(+) monocytes were cultured in the presence of different cytokine 
      combinations, human or porcine endothelial cells, and smooth muscle cells, and 
      analyzed for DC-specific antigen expression, antigen-presenting capacity, 
      cytokine and chemokine generation, and expression of Toll-like receptors by flow 
      cytometry and reverse transcription polymerase chain reaction. RESULTS: Human 
      monocytes express a DC-specific surface phenotype and efficiently present allo- 
      and xenoantigens to allogeneic T cells after co-culturing with allogeneic and 
      xenogeneic endothelial cells, respectively. Differentiation of monocytes under 
      different stimulating conditions is also accompanied by the up-regulation of 
      costimulatory molecules (CD40, CD80, CD86), adhesion molecules (CD54), human 
      leukocyte antigen (HLA)-DR, synthesis of cytokines tumor necrosis factor-alpha, 
      IL-12p70, IL-10, and differential expression of message for Toll-like receptors. 
      CONCLUSIONS: Porcine aortic endothelial cells can provide immunostimulatory 
      signals to human peripheral blood adherent monocytes similar to allogeneic 
      endothelial cells through the participation of innate immune mechanisms.
FAU - Manna, Partha Pratim
AU  - Manna PP
AD  - Departments of Surgery, Pathology, and Immunology, Washington University School 
      of Medicine, St. Louis, Missouri 63110, USA. kumart@msnotes.wustl.edu.
FAU - Steward, Nancy
AU  - Steward N
FAU - Lowell, Jeffrey
AU  - Lowell J
FAU - Mohanakumar, T
AU  - Mohanakumar T
LA  - eng
GR  - HL57796/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Drosophila Proteins)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptors)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Antigens, CD
MH  - CD40 Ligand/biosynthesis
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis
MH  - Dendritic Cells/physiology
MH  - *Drosophila Proteins
MH  - Endothelium, Vascular/*cytology
MH  - HLA-DR Antigens/biosynthesis
MH  - Humans
MH  - Immunoglobulins/biosynthesis
MH  - Lipopolysaccharide Receptors/*analysis
MH  - Membrane Glycoproteins/*biosynthesis
MH  - Monocytes/*physiology
MH  - Muscle, Smooth, Vascular/cytology
MH  - Receptors, Cell Surface/*biosynthesis
MH  - Toll-Like Receptors
MH  - Up-Regulation
MH  - CD83 Antigen
EDAT- 2002/08/02 10:00
MHDA- 2002/08/17 10:01
CRDT- 2002/08/02 10:00
PHST- 2002/08/02 10:00 [pubmed]
PHST- 2002/08/17 10:01 [medline]
PHST- 2002/08/02 10:00 [entrez]
AID - 10.1097/00007890-200207270-00016 [doi]
PST - ppublish
SO  - Transplantation. 2002 Jul 27;74(2):243-52. doi: 10.1097/00007890-200207270-00016.