PMID- 12152997
OWN - NLM
STAT- MEDLINE
DCOM- 20030113
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 43
IP  - 6
DP  - 2002 Jun
TI  - Monitoring of donor/recipient T-cell engraftment kinetics in myeloablative 
      allogeneic stem cell transplantation using short tandem repeat amplification from 
      cell lysates.
PG  - 1281-7
AB  - Molecular monitoring of donor/recipient T-cell kinetics early post-transplant can 
      provide clues to the immunological events that govern host-versus-graft reaction 
      (HVGR) and graft versus-host-disease (GVHD). We have previously used fluorescence 
      in situ hybridization (FISH) with X and Y probes to monitor recipient T (R-T) 
      cell clearance early after myeloablative allogeneic stem cell transplantation 
      (ASCT). We demonstrated that impaired clearance of residual host-T-cells in the 
      early days post-transplant was associated with graft rejection, while enhanced 
      clearance could be an indicator of increased donor anti-host alloreactivity and 
      predictive of acute GVHD. Although FISH is the most accurate quantitative 
      molecular tool for the determination of the exact donor/recipient-T-cell numbers 
      at any time points post-transplant, it has the disadvantage of being limited to 
      sex mismatched donor/recipient pairs. Our goal was to develop a molecular 
      approach that, irrespective of gender, would be comparable to FISH in accurately 
      determining host residual T-cell clearance after myeloablative conditioning for 
      ASCT. We have genotyped DNA from cell lysates using polymerase chain reaction 
      (PCR) amplification of short tandem repeats (STR) with fluorescently labeled 
      oligonucleotide primers, and used the Genescan 672 software for accurate 
      quantitative analysis of the amplified alleles. Here, we show that this approach 
      allowed us to achieve in T-cells accurate quantitative analyses of amplified 
      donor/recipient alleles in sex matched patients on days +5, +8 and +12 
      post-transplant, despite severe leukopenia.
FAU - Boumah, C E
AU  - Boumah CE
AD  - Section of Adult Hematology BMT, King Faisal Specialist Hospital and Research 
      Center, Riyadh, Kingdom of Saudi Arabia.
FAU - Meyer, B
AU  - Meyer B
FAU - Aljurf, M
AU  - Aljurf M
FAU - Bertilsson, P A B
AU  - Bertilsson PA
FAU - Pyle, R H
AU  - Pyle RH
FAU - Al-Hussein, K A F
AU  - Al-Hussein KA
FAU - Iqbal, A
AU  - Iqbal A
FAU - Gyger, M
AU  - Gyger M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alleles
MH  - Cell Survival
MH  - DNA/*genetics
MH  - Female
MH  - Graft Survival/*genetics
MH  - Humans
MH  - Leukemia/blood/therapy
MH  - Leukopenia/blood
MH  - Lymphocyte Count
MH  - Male
MH  - *Microsatellite Repeats
MH  - Myelodysplastic Syndromes/blood/therapy
MH  - *Peripheral Blood Stem Cell Transplantation
MH  - Polymerase Chain Reaction
MH  - Software
MH  - T-Lymphocytes/*cytology
MH  - Transplantation Chimera/blood
MH  - *Transplantation Conditioning
MH  - *Transplantation, Homologous
EDAT- 2002/08/03 10:00
MHDA- 2003/01/14 04:00
CRDT- 2002/08/03 10:00
PHST- 2002/08/03 10:00 [pubmed]
PHST- 2003/01/14 04:00 [medline]
PHST- 2002/08/03 10:00 [entrez]
AID - 10.1080/10428190290026349 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2002 Jun;43(6):1281-7. doi: 10.1080/10428190290026349.