PMID- 12153513
OWN - NLM
STAT- MEDLINE
DCOM- 20021001
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 106
IP  - 4
DP  - 2002 Aug
TI  - Effect of cell density on in vitro mouse immunoglobulin E production.
PG  - 503-10
AB  - In vitro immunoglobulin E (IgE) production was found to be sensitive to 
      increasing cell concentration in culture wells. While class switching to IgE is 
      intact as suggested by surface IgE staining, ELISPOT analysis provided evidence 
      that the differentiation of IgE committed B cells to the plasma cell stage was 
      arrested at high cell doses. In fact, splitting the cells at higher 
      concentrations after culture initiation increased IgE production. Cells plated at 
      higher doses were found to be more prone to apoptosis as assessed by Annexin 
      staining. Interestingly, inhibiting apoptosis by the use of the caspase inhibitor 
      DEVD significantly increased IgE levels implicating apoptosis in the preferential 
      deletion of IgE expressing cells. These data not only highlight the caveat 
      against using a single B-cell dose for IgE production in vitro but also suggest 
      for the first time a possible IgE regulatory mechanism mediated by cell density.
FAU - Rabah, Dania
AU  - Rabah D
AD  - Department of Microbiology and Immunology, Virginia Commonwealth University, 
      Richmond, VA 23298, USA.
FAU - Conrad, Daniel H
AU  - Conrad DH
LA  - eng
GR  - R01 AI018697/AI/NIAID NIH HHS/United States
GR  - R01 AI044163/AI/NIAID NIH HHS/United States
GR  - AI 18697/AI/NIAID NIH HHS/United States
GR  - AI 44163/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology
MH  - B-Lymphocytes/*immunology
MH  - Cell Count
MH  - Cell Culture Techniques
MH  - Cell Differentiation/immunology
MH  - Cell Survival/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoglobulin Class Switching
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Immunoglobulin M/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
PMC - PMC1782743
EDAT- 2002/08/03 10:00
MHDA- 2002/10/03 04:00
PMCR- 2003/08/01
CRDT- 2002/08/03 10:00
PHST- 2002/08/03 10:00 [pubmed]
PHST- 2002/10/03 04:00 [medline]
PHST- 2002/08/03 10:00 [entrez]
PHST- 2003/08/01 00:00 [pmc-release]
AID - 1435 [pii]
AID - 10.1046/j.1365-2567.2002.01435.x [doi]
PST - ppublish
SO  - Immunology. 2002 Aug;106(4):503-10. doi: 10.1046/j.1365-2567.2002.01435.x.