PMID- 12154203
OWN - NLM
STAT- MEDLINE
DCOM- 20020904
LR  - 20190513
IS  - 1462-0324 (Print)
IS  - 1462-0324 (Linking)
VI  - 41
IP  - 8
DP  - 2002 Aug
TI  - Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in 
      leucocytes from patients with systemic sclerosis.
PG  - 869-75
AB  - OBJECTIVE: To determine the expression of molecular markers of prostanoid/fatty 
      acid signalling in leucocytes of patients with systemic sclerosis (SSc). METHODS: 
      Gene expression in patient leucocytes was analysed using real-time fluorescence 
      reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha 
      (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), 
      monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated 
      receptors (PPAR) alpha, delta and gamma, low-density lipoprotein-associated 
      lipoprotein lipase A(2) (LDL-PLA(2)), apolipoprotein E (apoE) and cholesterol 
      27-hydroxylase (CYP27). RESULTS: Both TNF-alpha and TXAS showed an increase in 
      mean expression in the diseased group (6.3-fold and 5.6-fold respectively, 
      P<0.0001). These two markers, along with CYP27, PPARgamma and apoE, provided 
      predictive markers for the development of carotid artery disease within the SSc 
      patient population. CONCLUSION: The elevated levels of TNF-alpha and thromboxane 
      seen in SSc patient sera are paralleled by increases in the expression of the 
      appropriate genes in leucocytes. This method will allow us to screen for a large 
      number of candidate markers of disease in order to increase our understanding of 
      the processes underlying the pathology of SSc.
FAU - Young, V
AU  - Young V
AD  - Biomedical Research Centre, Department of Medicine, Ninewells Hospital and 
      Medical School, University of Dundee, UK.
FAU - Ho, M
AU  - Ho M
FAU - Vosper, H
AU  - Vosper H
FAU - Belch, J J F
AU  - Belch JJ
FAU - Palmer, C N A
AU  - Palmer CN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 5.3.99.5 (Thromboxane-A Synthase)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - *Gene Expression Regulation
MH  - Genetic Markers
MH  - Humans
MH  - Leukocytes/enzymology/*immunology
MH  - Reference Values
MH  - Scleroderma, Systemic/enzymology/*genetics/immunology
MH  - Thromboxane-A Synthase/blood/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2002/08/03 10:00
MHDA- 2002/09/06 10:01
CRDT- 2002/08/03 10:00
PHST- 2002/08/03 10:00 [pubmed]
PHST- 2002/09/06 10:01 [medline]
PHST- 2002/08/03 10:00 [entrez]
AID - 10.1093/rheumatology/41.8.869 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2002 Aug;41(8):869-75. doi: 10.1093/rheumatology/41.8.869.