PMID- 12164258
OWN - NLM
STAT- MEDLINE
DCOM- 20021107
LR  - 20231213
IS  - 1056-8719 (Print)
IS  - 1056-8719 (Linking)
VI  - 46
IP  - 1
DP  - 2001 Jul-Aug
TI  - A sensitive method for determining levels of 
      [-]-2,5,-dimethoxy-4-methylamphetamine in the brain tissue.
PG  - 37-43
AB  - INTRODUCTION: Indolamine and phenethylamine hallucinogens are drugs of abuse and, 
      as well, mimic some aspects of idiopathic psychosis. To assist in investigating 
      the mechanisms of action of (-)2,5-dimethoxy4-methylamphetamine ([-]-DOM), a 
      member of the phenethylamine class of serotonergic hallucinogens, a sensitive and 
      precise method for determining its levels in the brain tissue is required. 
      METHODS: We now describe a method for determining nanogram quantities of [-]-DOM 
      in the rat brain tissue using D-amphetamine as an intemal standard. The method 
      employs solvent extraction with toluene and derivatization with trifluoroacetic 
      acid anhydride (TFAA) followed by analysis using gas chromatography-mass 
      spectrometry (GS-MS) in the selective ion monitoring (SIM) mode. RESULTS: With 
      SIM detection, our overall recoveries were greater than 90%. The method was 
      reliable in terms of within-day and between-day precision, accuracy, and 
      linearity. The procedure was applied to animal subjects to determine the in vivo 
      [-]-DOM brain levels following intraperitoneal (ip) administration. Our findings 
      indicate that peak levels of [-]-DOM do not coincide with the 75-min pretreatment 
      time established by drug-induced stimulus control. DISCUSSION: This study 
      demonstrates a sensitive and precise analytical method for the determination of 
      [-]-DOM levels in the rat brain following systemic administration of behaviorally 
      relevant doses.
FAU - Eckler, J R
AU  - Eckler JR
AD  - Department of Pharmacology and Toxicology, School of Medicine and Biomedical 
      Sciences, State University of New York at Buffalo, 14214-3000, USA. 
      eckler@buffalo.edu
FAU - Greizerstein, H
AU  - Greizerstein H
FAU - Rabin, R A
AU  - Rabin RA
FAU - Winter, J C
AU  - Winter JC
LA  - eng
GR  - DA 03385/DA/NIDA NIH HHS/United States
GR  - DA 13920-01/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pharmacol Toxicol Methods
JT  - Journal of pharmacological and toxicological methods
JID - 9206091
RN  - 0 (Hallucinogens)
RN  - 15588-95-1 (DOM 2,5-Dimethoxy-4-Methylamphetamine)
RN  - TZ47U051FI (Dextroamphetamine)
SB  - IM
MH  - DOM 2,5-Dimethoxy-4-Methylamphetamine/*pharmacokinetics
MH  - Animals
MH  - Brain/*metabolism
MH  - Dextroamphetamine/pharmacokinetics
MH  - Dose-Response Relationship, Drug
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hallucinogens/*pharmacokinetics
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reproducibility of Results
EDAT- 2002/08/08 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/08/08 10:00
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/08/08 10:00 [entrez]
AID - S1056-8719(02)00159-4 [pii]
AID - 10.1016/s1056-8719(02)00159-4 [doi]
PST - ppublish
SO  - J Pharmacol Toxicol Methods. 2001 Jul-Aug;46(1):37-43. doi: 
      10.1016/s1056-8719(02)00159-4.