PMID- 12165275
OWN - NLM
STAT- MEDLINE
DCOM- 20021010
LR  - 20220321
IS  - 1521-6616 (Print)
IS  - 1521-6616 (Linking)
VI  - 104
IP  - 2
DP  - 2002 Aug
TI  - Reduction in the circulating pDC1/pDC2 ratio and impaired function of ex 
      vivo-generated DC1 in chronic hepatitis B infection.
PG  - 138-50
AB  - Dendritic cells (DCs) induce and regulate T-cell-mediated immune responses. 
      Circulating precursor (p)DC1 and pDC2 from patients with chronic hepatitis B 
      virus (HBV) infection were quantified by flow cytometry. To assess their 
      function, DC1 were cultured from patients and compared to those of healthy 
      volunteers. HBV patients exhibited a significant decrease in the proportion of 
      freshly isolated pDC1 to pDC2. DC1 propagated from patients showed lower 
      expression of costimulatory molecules and impaired allostimulatory capacity in 
      comparison to controls. After exposure to proinflammatory cytokines, expression 
      of costimulatory molecules, secretion of interleukin-12 (IL-12) and 
      allostimulatory properties increased, but capacity for T-cell stimulation and 
      IL-12 production remained inferior to that of control DCs. HBV-DNA was amplified 
      by polymerase chain reaction in DC1 cultured from all patients. Viral particles 
      were visible in DC1 by electron microscopy. These results suggest that 
      intracellular presence of HBV impairs DC1 functional maturation and subsequent 
      deficits in T-lymphocyte activation may contribute to viral persistence.
FAU - Beckebaum, Susanne
AU  - Beckebaum S
AD  - Thomas E Starlz Transplantation Institute, University of Pittsburgh Medical 
      Center, Pittsburgh, PA 15213, USA. susanne.beckebaum@uni-essen.de
FAU - Cicinnati, Vito R
AU  - Cicinnati VR
FAU - Dworacki, Grzegorz
AU  - Dworacki G
FAU - Muller-Berghaus, Jan
AU  - Muller-Berghaus J
FAU - Stolz, Donna
AU  - Stolz D
FAU - Harnaha, Jo
AU  - Harnaha J
FAU - Whiteside, Theresa L
AU  - Whiteside TL
FAU - Thomson, Angus W
AU  - Thomson AW
FAU - Lu, Lina
AU  - Lu L
FAU - Fung, John J
AU  - Fung JJ
FAU - Bonham, C Andrew
AU  - Bonham CA
LA  - eng
GR  - CA 73743/CA/NCI NIH HHS/United States
GR  - DK 49745/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (DNA, Viral)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cell Division
MH  - Cells, Cultured
MH  - DNA, Viral/analysis/blood
MH  - Dendritic Cells/drug effects/*immunology/virology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hepatitis B virus/genetics/isolation & purification
MH  - Hepatitis B, Chronic/blood/*immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-12/analysis
MH  - Interleukin-4/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Time Factors
EDAT- 2002/08/08 10:00
MHDA- 2002/10/11 04:00
CRDT- 2002/08/08 10:00
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2002/10/11 04:00 [medline]
PHST- 2002/08/08 10:00 [entrez]
AID - S1521661602952459 [pii]
AID - 10.1006/clim.2002.5245 [doi]
PST - ppublish
SO  - Clin Immunol. 2002 Aug;104(2):138-50. doi: 10.1006/clim.2002.5245.