PMID- 12165445 OWN - NLM STAT- MEDLINE DCOM- 20020820 LR - 20231213 IS - 0165-4608 (Print) IS - 0165-4608 (Linking) VI - 136 IP - 1 DP - 2002 Jul 1 TI - Cryptic t(12;15)(p13;q26) producing the ETV6-NTRK3 fusion gene and no loss of IGF2 imprinting in congenital mesoblastic nephroma with trisomy 11: fluorescence in situ hybridization and IGF2 allelic expression analysis. PG - 10-6 AB - In the present fluorescence in situ hybridization (FISH) study of six congenital mesoblastic nephromas (CMNs) using ETV6 and NTRK3 probes as well as a chromosome 15 painting probe, we identified a cryptic reciprocal translocation, t(12;15)(p13;q26), in one tumor, and an insertion, ins(12;15)(p13;q22q26), in another that were not previously identified by cytogenetic analysis. An interphase FISH study with the same probes detected the ETV6-NTRK3 fusion signal in all three cellular or mixed type tumors, but not in all three classical type tumors. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis detected the ETV6-NTRK3 fusion transcript in the three cellular or mixed type tumors, but not in the three classical type tumors. FISH analysis using a chromosome 11-centromere probe detected trisomy or tetrasomy 11 in all three tumors with the ETV6-NTRK3 fusion signal. To clarify whether IGF2, a paternally expressed gene on chromosome 11, has a certain role in the tumorigenic process of CMN through a loss of imprinting (LOI), we studied IGF2 allelic expression. We found no LOI in two cellular or mixed type tumors or in two classical type tumors, and concluded that the role of the LOI of IGF2 is not essential for the development and progression of CMN with or without trisomy 11. Furthermore, we showed no rearrangements of the MLL gene, which is frequently rearranged in acute leukemia with +11 in the three CMN tumors with +11. FAU - Watanabe, Naoki AU - Watanabe N AD - Departments of Cancer Chemotherapy and Medicine, Saitama Cancer Center Hospital, Ina, Saitama 362-0806, Japan. FAU - Kobayashi, Hirofumi AU - Kobayashi H FAU - Hirama, Toshinori AU - Hirama T FAU - Kikuta, Atsushi AU - Kikuta A FAU - Koizumi, Shoichi AU - Koizumi S FAU - Tsuru, Tomomitsu AU - Tsuru T FAU - Kaneko, Yasuhiko AU - Kaneko Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cancer Genet Cytogenet JT - Cancer genetics and cytogenetics JID - 7909240 RN - 0 (DNA-Binding Proteins) RN - 0 (KMT2A protein, human) RN - 0 (Proto-Oncogene Proteins c-ets) RN - 0 (RNA, Messenger) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Repressor Proteins) RN - 0 (Transcription Factors) RN - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein) RN - 67763-97-7 (Insulin-Like Growth Factor II) RN - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase) RN - EC 2.7.10.1 (Receptor, trkC) SB - IM MH - Alleles MH - *Chromosomes, Human, Pair 11 MH - Chromosomes, Human, Pair 12 MH - Chromosomes, Human, Pair 15 MH - DNA-Binding Proteins/genetics/metabolism MH - Female MH - Gene Expression MH - Genomic Imprinting MH - Histone-Lysine N-Methyltransferase MH - Humans MH - In Situ Hybridization, Fluorescence MH - Infant MH - Infant, Newborn MH - Insulin-Like Growth Factor II/biosynthesis/*genetics MH - Karyotyping MH - Kidney Neoplasms/*genetics MH - Male MH - Myeloid-Lymphoid Leukemia Protein MH - Nephroma, Mesoblastic/*genetics MH - Proto-Oncogene Proteins c-ets MH - *Proto-Oncogenes MH - RNA, Messenger/analysis MH - Receptor, trkC/genetics/metabolism MH - Recombinant Fusion Proteins/metabolism MH - Repressor Proteins/genetics/metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - *Transcription Factors MH - *Translocation, Genetic MH - Trisomy MH - ETS Translocation Variant 6 Protein EDAT- 2002/08/08 10:00 MHDA- 2002/08/21 10:01 CRDT- 2002/08/08 10:00 PHST- 2002/08/08 10:00 [pubmed] PHST- 2002/08/21 10:01 [medline] PHST- 2002/08/08 10:00 [entrez] AID - S0165460802005290 [pii] AID - 10.1016/s0165-4608(02)00529-0 [doi] PST - ppublish SO - Cancer Genet Cytogenet. 2002 Jul 1;136(1):10-6. doi: 10.1016/s0165-4608(02)00529-0.