PMID- 12165528
OWN - NLM
STAT- MEDLINE
DCOM- 20020903
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 169
IP  - 4
DP  - 2002 Aug 15
TI  - Reversal of coccidioidal anergy in vitro by dendritic cells from patients with 
      disseminated coccidioidomycosis.
PG  - 2020-5
AB  - Coccidioides immitis is a pathogenic, dimorphic fungus found in the southwestern 
      United States and is the causative agent of coccidioidomycosis. Extrathoracic 
      dissemination of coccidioidomycosis is associated with a lack of cellular 
      immunity. Dendritic cells (DCs) have been shown to initiate and modulate cellular 
      immune responses. To determine whether DCs could modulate or initiate the immune 
      response in this disease, monocyte-derived DCs were generated from coccidioidal 
      Ag nonresponsive patients with disseminated coccidioidomycosis and healthy 
      nonimmune individuals. DCs generated from both groups demonstrated phenotypes 
      characteristic of DCs and stimulated strong allogeneic MLR. DCs from patients and 
      healthy nonimmune individuals pulsed with the coccidioidal Ag preparation T27K 
      induced lymphocyte proliferation. Mature DCs were much more efficient than 
      immature DCs in these stimulations. Furthermore, restimulation of T27K-primed 
      PBMC with Ag-pulsed DCs generated a C. immitis-specific cellular immune response 
      in PBMC from patients with disseminated coccidioidomycosis as well as healthy 
      nonimmune individuals. These results show that 1) DCs have the capacity to 
      stimulate specific cellular immune responses from patients with disseminated 
      coccidioidomycosis who are nonresponsive to coccidioidal Ag and healthy nonimmune 
      individuals in vitro; 2) DCs can be used to screen coccidioidal Ags as candidates 
      for human vaccine development; and 3) DC therapy may be useful in the treatment 
      of disseminated coccidioidomycosis.
FAU - Richards, John O
AU  - Richards JO
AD  - Department of Microbiology and Immunology, Arizona Health Sciences Center, 
      Tucson, AZ 85724, USA.
FAU - Ampel, Neil M
AU  - Ampel NM
FAU - Lake, Douglas F
AU  - Lake DF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Fungal)
SB  - IM
MH  - Antigens, Fungal/administration & dosage
MH  - Clonal Anergy
MH  - Coccidioides/*immunology
MH  - Coccidioidomycosis/*immunology/therapy
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Immunity, Cellular
MH  - Immunotherapy
MH  - In Vitro Techniques
MH  - Lymphocyte Activation
EDAT- 2002/08/08 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/08/08 10:00
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/08/08 10:00 [entrez]
AID - 10.4049/jimmunol.169.4.2020 [doi]
PST - ppublish
SO  - J Immunol. 2002 Aug 15;169(4):2020-5. doi: 10.4049/jimmunol.169.4.2020.