PMID- 12165536
OWN - NLM
STAT- MEDLINE
DCOM- 20020903
LR  - 20220129
IS  - 0022-1767 (Print)
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 169
IP  - 4
DP  - 2002 Aug 15
TI  - Annexin 1 modulates monocyte-endothelial cell interaction in vitro and cell 
      migration in vivo in the human SCID mouse transplantation model.
PG  - 2085-92
AB  - The effect of the glucocorticoid inducible protein annexin 1 (ANXA1) on the 
      process of monocytic cell migration was studied using transfected U937 cells 
      expressing variable protein levels. An antisense (AS) (36.4AS; approximately 50% 
      less ANXA1) and a sense (S) clone (15S; overexpressing the bioactive 24-kDa 
      fragment) together with the empty plasmid CMV clone were obtained and compared 
      with wild-type U937 cells in various models of cell migration in vitro and in 
      vivo. 15S-transfected U937 cells displayed a reduced (50%) degree of 
      trans-endothelial migration in response to stromal cell-derived factor-1alpha 
      (CXC chemokine ligand 12 (CXCL12)). In addition, the inhibitory role of 
      endogenous ANXA1 on U937 cell migration in vitro was confirmed by the 
      potentiating effect of a neutralizing anti-ANXA1 serum. Importantly, 
      overexpression of ANXA1 in clone 15S inhibited the extent of cell migration into 
      rheumatoid synovial grafts transplanted into SCID mice. ANXA1 inhibitory effects 
      were not due to modifications in adhesion molecule or CXCL12 receptor (CXCR4) 
      expression as shown by the similar amounts of surface molecules found in 
      transfected and wild-type U937 cells. Likewise, an equal chemotactic response to 
      CXCL12 in vitro excluded an intrinsic defect in cell motility in clones 15S and 
      36.4AS. These data strongly support the notion that ANXA1 critically interferes 
      with a leukocyte endothelial step essential for U937 cell, and possibly monocyte, 
      transmigration both in vitro and in vivo.
FAU - Perretti, Mauro
AU  - Perretti M
AD  - William Harvey Research Institute, London, United Kingdom. M.Perretti@qmul.ac.uk
FAU - Ingegnoli, Francesca
AU  - Ingegnoli F
FAU - Wheller, Samantha K
AU  - Wheller SK
FAU - Blades, Mark C
AU  - Blades MC
FAU - Solito, Egle
AU  - Solito E
FAU - Pitzalis, Costantino
AU  - Pitzalis C
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - 15718/ARC_/Arthritis Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Annexin A1)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl12 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Annexin A1/antagonists & inhibitors/genetics/*physiology
MH  - Cell Movement/physiology
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/pharmacology
MH  - Chemotaxis
MH  - Endothelium, Vascular/cytology/drug effects/*physiology
MH  - Gene Expression
MH  - Humans
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, SCID
MH  - Molecular Sequence Data
MH  - Monocytes/drug effects/*physiology
MH  - Synovial Membrane/transplantation
MH  - Transfection
MH  - Transplantation, Heterologous
MH  - U937 Cells
PMC - PMC4340507
MID - EMS28034
OID - NLM: EMS28034
EDAT- 2002/08/08 10:00
MHDA- 2002/09/11 10:01
PMCR- 2015/02/25
CRDT- 2002/08/08 10:00
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/08/08 10:00 [entrez]
PHST- 2015/02/25 00:00 [pmc-release]
AID - 10.4049/jimmunol.169.4.2085 [doi]
PST - ppublish
SO  - J Immunol. 2002 Aug 15;169(4):2085-92. doi: 10.4049/jimmunol.169.4.2085.