PMID- 12165797
OWN - NLM
STAT- MEDLINE
DCOM- 20030225
LR  - 20081121
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 10
IP  - 3
DP  - 2002 Sep
TI  - Cultivation of fetal erythroid precursors from maternal blood: isolation and 
      characterization by PCR and FISH.
PG  - 257-61
AB  - Cultivation of fetal progenitor cells from maternal blood offers the opportunity 
      to produce sufficient fetal cells for prenatal molecular genetic and cytogenetic 
      analysis. For in vitro cultivation, 10 ml of blood was collected from 22 women 
      carrying a male fetus. After triple-density gradient centrifugation, the 
      mononucleated cells were cultivated for 10 to 14 days in special hematopoietic 
      growth medium. Red and white colored cell colonies were individually collected by 
      micromanipulation. A representative sample from each colony was characterized by 
      chromosome Y-specific polymerase chain reaction (PCR) systems. The remaining 
      cells of the Y-positive colonies were used to perform chromosome preparations and 
      fluorescence in situ hybridization (FISH) to detect XY-positive interphase nuclei 
      and metaphases. Y-positive signals could be detected in 15 (68%) of the 22 
      analyzed blood samples. With SRY PCR 10.5% (40/379) of the collected red colonies 
      were determined to be of fetal origin and 6.1% (32/522) of the colonies analyzed 
      by amelogenin PCR were Y-positive. All collected white cell colonies were 
      Y-negative. FISH analyses of PCR-positive colonies revealed that less than 30% of 
      the cells within a colony are of fetal origin and reflect more precisely the 
      actual situation within a single colony. Moreover the successful preparation of 
      fetal metaphases in non-invasive prenatal diagnosis is presented.
FAU - Hohmann, Harald
AU  - Hohmann H
AD  - Institute of Human Genetics and Anthropology, University of Jena, Germany.
FAU - Michel, Susanne
AU  - Michel S
FAU - Reiber, Wolfgang
AU  - Reiber W
FAU - Kahler, Christiane
AU  - Kahler C
FAU - Claussen, Uwe
AU  - Claussen U
FAU - Von Eggeling, Ferdinand
AU  - Von Eggeling F
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
SB  - IM
MH  - *Chromosomes, Human, Y
MH  - Erythroid Precursor Cells/*ultrastructure
MH  - Female
MH  - Fetus
MH  - Gestational Age
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Metaphase
MH  - Polymerase Chain Reaction/*methods
MH  - Pregnancy
MH  - Prenatal Diagnosis/methods
EDAT- 2002/08/08 10:00
MHDA- 2003/02/26 04:00
CRDT- 2002/08/08 10:00
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2003/02/26 04:00 [medline]
PHST- 2002/08/08 10:00 [entrez]
PST - ppublish
SO  - Int J Mol Med. 2002 Sep;10(3):257-61.