PMID- 12165826
OWN - NLM
STAT- MEDLINE
DCOM- 20020916
LR  - 20061115
IS  - 1432-2218 (Electronic)
IS  - 0930-2794 (Linking)
VI  - 16
IP  - 7
DP  - 2002 Jul
TI  - MCP-1 is highly expressed in peritoneum following midline laparotomy with 
      peritoneal abrasion in a murine model.
PG  - 1079-82
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1), a CC chemokine, is a 
      potent attractant of monocytes both in vitro and in vivo. However, its role in 
      the repair of peritoneal injury is not well established. This study characterizes 
      MCP-1 expression in surgical wounds following peritoneal abrasion in a murine 
      model. METHODS: Twenty-five C57 BL6 female mice underwent a 2-cm midline 
      laparotomy with mechanical abrasion of the right peritoneal wall. The mice were 
      sacrificed at various times ranging from 0 to 7 days. Hemotoxylin and eosin 
      stained sections and tissue extracts were made using peritoneal samples from 
      abraded and unabraded areas in each mouse. An enzyme-linked immunosorbent assay 
      was performed on the specimens to quantitate MCP-1 expression. Values were 
      compared using a t-test. RESULTS: At baseline, there was minimal expression of 
      MCP-1 (<5 pg/mg protein). Following surgery, MCP-1 levels at abraded sites were 
      significantly higher than those at both baseline and unabraded sites at all times 
      up to a week following surgery. Histologic evaluation revealed peritoneal 
      thickening and leukocytic infiltration of only abraded surfaces. CONCLUSION: 
      MCP-1 is highly expressed in peritoneum following laparotomy with peritoneal 
      abrasion. Elevations in MCP-1 levels are identified within 6 h of surgery and 
      persist for up to 1 week. The histologic differences between abraded and 
      unabraded areas may be attributable to differences in MCP-1 expression. Further 
      studies using recombinant MCP-1 and anti-MCP-1 antibody may elucidate this 
      relationship.
FAU - Brodsky, J A
AU  - Brodsky JA
AD  - Minimally Invasive Surgery Center and Department of Immunology, the Cleveland 
      Clinic Foundation, Cleveland, OH 44195, USA. brodyf@ccf.org
FAU - Brody, F J
AU  - Brody FJ
FAU - Endlich, B
AU  - Endlich B
FAU - Armstrong, D A
AU  - Armstrong DA
FAU - Ponsky, J L
AU  - Ponsky JL
FAU - Hamilton, I A
AU  - Hamilton IA
LA  - eng
PT  - Journal Article
DEP - 20020409
PL  - Germany
TA  - Surg Endosc
JT  - Surgical endoscopy
JID - 8806653
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Cell Movement/physiology
MH  - Chemokine CCL2/*biosynthesis/metabolism/physiology
MH  - Female
MH  - Laparotomy/*methods
MH  - Leukocytes/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Peritoneal Cavity/pathology/*surgery
MH  - Peritoneum/*metabolism/pathology/*surgery
MH  - Up-Regulation/physiology
MH  - Wound Healing/physiology
EDAT- 2002/08/08 10:00
MHDA- 2002/09/17 10:01
CRDT- 2002/08/08 10:00
PHST- 2001/04/26 00:00 [received]
PHST- 2001/12/19 00:00 [accepted]
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2002/09/17 10:01 [medline]
PHST- 2002/08/08 10:00 [entrez]
AID - 10.1007/s00464-001-8335-z [doi]
PST - ppublish
SO  - Surg Endosc. 2002 Jul;16(7):1079-82. doi: 10.1007/s00464-001-8335-z. Epub 2002 
      Apr 9.