PMID- 12165932
OWN - NLM
STAT- MEDLINE
DCOM- 20021008
LR  - 20131121
IS  - 1046-199X (Print)
IS  - 1046-199X (Linking)
VI  - 13
IP  - 3
DP  - 2002 Sep
TI  - Sodium metasilicate hypersensitivity in BALB/c mice.
PG  - 133-9
AB  - BACKGROUND: Sodium metasilicate (SMS) is a key ingredient for a number of 
      industrial and consumer products. Although little is known about potential for 
      this chemical to cause allergic reactions, a similar silicate compound, sodium 
      silicate, was reported to elicit IgE-mediated contact urticaria. OBJECTIVE: The 
      aim of this study was to evaluate the potential for sodium metasilicate to elicit 
      an allergic response in female BALB/c mice after dermal exposure. METHODS: The 
      primary irritancy assay (IA), local lymph node assay (LLNA), and a mouse ear 
      swelling test (MEST) were used to evaluate the hypersensitivity response elicited 
      by SMS exposure. An evaluation of lymph node subpopulations, cytokine mRNA 
      expression, and serum IgE levels was also conducted. RESULTS: SMS caused 
      significant dermal irritation at concentrations >or=6% and an allergic response 
      after mice were sensitized with 4% SMS then challenged with 6% SMS in the MEST. 
      Lymph node cell proliferation was not observed in the LLNA after treatment with 
      SMS (2% to 6% SMS). Increases in lymph node cellularity, the percentage of B 
      cells, and the expression of certain cytokine mRNAs were observed in mice treated 
      with SMS. Changes in the concentration of serum IgE after SMS treatment, however, 
      were not observed. CONCLUSIONS: SMS appears to elicit a chemical hypersensitivity 
      response in mice, as indicated by the MEST, but not by the LLNA. Increases in 
      auricular lymph node cellularity, the percentage of B cells, and certain cytokine 
      mRNAs support classifying SMS as a weak chemical allergen.
CI  - Copyright 2002, Elsevier Science (USA). All rights reserved.
FAU - Karrow, N A
AU  - Karrow NA
AD  - Department of Pharmacology and Toxicology, Virginia Commonwealth University, 
      Richmond, VA 23298, USA.
FAU - Guo, T L
AU  - Guo TL
FAU - Leffel, E K
AU  - Leffel EK
FAU - Zhang, L X
AU  - Zhang LX
FAU - McCay, J A
AU  - McCay JA
FAU - Germolec, D R
AU  - Germolec DR
FAU - White, K L Jr
AU  - White KL Jr
LA  - eng
GR  - ES55387/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Canada
TA  - Am J Contact Dermat
JT  - American journal of contact dermatitis : official journal of the American Contact 
      Dermatitis Society
JID - 9100472
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Silicates)
RN  - 052612U92L (sodium metasilicate)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - *Allergens
MH  - Animals
MH  - Cell Count
MH  - Cell Division
MH  - Cytokines/genetics/metabolism
MH  - Dermatitis, Contact/diagnosis/etiology
MH  - Dermatitis, Irritant/etiology
MH  - Dinitrofluorobenzene/toxicity
MH  - Ear, External
MH  - Edema/chemically induced
MH  - Female
MH  - Hypersensitivity/*diagnosis/etiology
MH  - Immunoglobulin E/blood
MH  - Local Lymph Node Assay
MH  - Lymph Nodes/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Messenger/analysis
MH  - Silicates/*toxicity
EDAT- 2002/08/08 10:00
MHDA- 2002/10/09 04:00
CRDT- 2002/08/08 10:00
PHST- 2002/08/08 10:00 [pubmed]
PHST- 2002/10/09 04:00 [medline]
PHST- 2002/08/08 10:00 [entrez]
AID - S1046199X02000064 [pii]
PST - ppublish
SO  - Am J Contact Dermat. 2002 Sep;13(3):133-9.