PMID- 12166811
OWN - NLM
STAT- MEDLINE
DCOM- 20030107
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 26
IP  - 5
DP  - 2002 Jul-Aug
TI  - Rapid simultaneous determination of amphetamine, methamphetamine, 
      3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 
      3,4-methylenedioxyethylamphetamine in urine by solid-phase extraction and GC-MS: 
      a method optimized for high-volume laboratories.
PG  - 253-61
AB  - To facilitate analysis of high sample volumes, an extraction, derivatization and 
      gas chromatographic-mass spectrometric analysis method was developed to 
      simultaneously determine amphetamine (AMP), methamphetamine (MAMP), 
      3,4-methylenedioxyamphetamine (MDA) 3,4-methylenedioxymethamphetamine (MDMA), and 
      3,4-methylenedioxyethylamphetamine (MDEA) in urine. This method utilized a 
      positive-pressure manifold cation-exchange polymer-based solid-phase extraction 
      followed by elution directly into automated liquid sampler (ALS) vials. Rapid 
      derivatization was accomplished using heptafluorobutyric anhydride (HFBA). 
      Recoveries averaged 90% or greater for each of the compounds. Limits of detection 
      were 62.5 ng/mL (AMP and MDEA), 15.6 ng/mL (MAMP), and 31.3 ng/mL (MDA and MDMA) 
      using a 2-mL sample volume. The method was linear to 5000 ng/mL for all compounds 
      using MDMA-d5 and MAMP-d14 as internal standards. Over 200 human urine samples 
      previously determined to contain the target analytes were analyzed using the 
      method. Excellent agreement was seen with previous quantitations. The method was 
      challenged with 75 potentially interfering compounds and no interferences were 
      seen. These interfering compounds included ephedrine, pseudoephedrine, 
      phenylpropanolamine, and phenethylamine. The method resulted in dramatic 
      reductions in processing time and waste production.
FAU - Stout, Peter R
AU  - Stout PR
AD  - Navy Drug Screening Laboratory, Naval Air Station, Jacksonville, Florida 32212, 
      USA.
FAU - Horn, Carl K
AU  - Horn CK
FAU - Klette, Kevin L
AU  - Klette KL
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Hallucinogens)
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - CK833KGX7E (Amphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*urine
MH  - Amphetamine/*urine
MH  - Central Nervous System Stimulants/*urine
MH  - Gas Chromatography-Mass Spectrometry/methods/standards
MH  - Hallucinogens/*urine
MH  - Humans
MH  - Methamphetamine/analogs & derivatives/*urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*urine
MH  - Reference Values
MH  - Specimen Handling
EDAT- 2002/08/09 10:00
MHDA- 2003/01/08 04:00
CRDT- 2002/08/09 10:00
PHST- 2002/08/09 10:00 [pubmed]
PHST- 2003/01/08 04:00 [medline]
PHST- 2002/08/09 10:00 [entrez]
AID - 10.1093/jat/26.5.253 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2002 Jul-Aug;26(5):253-61. doi: 10.1093/jat/26.5.253.