PMID- 12167103
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20190916
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 52
IP  - 7
DP  - 2002 Jul
TI  - Detection of c-erbB-2 (HER-2/neu) amplification in breast carcinoma by 
      fluorescence in situ hybridization on tissue sections and imprinted cells.
PG  - 451-7
AB  - Abnormalities in c-erbB-2 have attracted a great deal of attention. Treatment 
      using an antibody against the c-erbB-2 gene product is effective against breast 
      cancers with amplification and/or overexpression of c-erbB-2. There is an urgent 
      need to establish methodology for selecting patients who would benefit from this 
      therapy. A total of 235 breast carcinomas were examined for c-erbB-2 protein 
      overexpression by immunohistochemistry. Tissue sections with discernible 
      immunostaining from 52 tumors, 70 negative tumors and smear imprints from 35 
      patients were examined by dual-color fluorescence in situ hybridization (FISH) 
      using probes specific for c-erbB-2 and the centromeric region of chromosome 17. 
      The concordance between gene amplification and protein overexpression was 95.7%. 
      When the findings of the two FISH preparation techniques were compared, no 
      discrepancies were found in 24 of the tumors. However, differences were seen in 
      eight cases. In six of these cases the differences did not affect the presence or 
      absence of amplification, but in the other two cases, considered to show 
      low-level amplification on paraffin sections, polysomy 17 was detected instead. 
      It was concluded that FISH is an excellent tool to detect gene amplification in 
      particular, and FISH on touch imprints is a useful adjunct to differentiate 
      between low-level amplification and polysomy 17.
FAU - Kunitomo, KazuyoshI
AU  - Kunitomo K
AD  - Department of Pathology, Yamanashi Medical University, Yamanashi, Japan, 
      Department of Surgery, Yamanashi Medical University, Yamanashi, Japan.
FAU - Takehana, Takuo
AU  - Takehana T
FAU - Inoue, Shingo
AU  - Inoue S
FAU - Fujii, Hideki
AU  - Fujii H
FAU - Suzuki, Shioto
AU  - Suzuki S
FAU - Matsumoto, Yoshiro
AU  - Matsumoto Y
FAU - Ooi, Akishi
AU  - Ooi A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
SB  - IM
MH  - Breast Neoplasms/*genetics/metabolism
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Diagnosis, Differential
MH  - *Gene Amplification
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
MH  - Specimen Handling
EDAT- 2002/08/09 10:00
MHDA- 2003/02/14 04:00
CRDT- 2002/08/09 10:00
PHST- 2002/08/09 10:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/08/09 10:00 [entrez]
AID - 1374 [pii]
AID - 10.1046/j.1440-1827.2002.01374.x [doi]
PST - ppublish
SO  - Pathol Int. 2002 Jul;52(7):451-7. doi: 10.1046/j.1440-1827.2002.01374.x.