PMID- 12167999
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 0582-9879 (Print)
IS  - 0582-9879 (Linking)
VI  - 30
IP  - 6
DP  - 1998
TI  - A prostate-specific Protein Factor Inhibits the Interaction of Androgen Receptors 
      with Hormone Response Elements.
PG  - 618-622
AB  - The fragments of the androgen receptor (amino acids: 359-732) and of the 
      glucocorticoid receptor (amino acids: 396-548) were expressed in E. coli as 
      fusion proteins with GST. Both fusion proteins, denoted GST-AR and GST-GR, 
      contained the DNA-binding domain and some flanking amino acids. In gel 
      retardation assay both fusion proteins could bind the androgen/glucocorticoid 
      response element (ARE/GRE). We found that both cytosol and nuclear extracts from 
      rat ventral prostate (v.p), but not from other source tested could abolish the 
      interaction of GST-AR and GST-GR with ARE/GRE (from C3 (1) gene and MMTV LTR). 
      The inhibition was androgen-dependent and sensitive to temperature and trypsin 
      treatment. It implies that a protein inhibitor was present in the rat ventral 
      prostate.
FAU - Xie, Fang
AU  - Xie F
AD  - State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, 
      the Chinese Academy of Sciences, Shanghai 200031, China. yonglz@sunm.shcnc.ac.cn
FAU - Liu, Qiang
AU  - Liu Q
FAU - Liu, Jun
AU  - Liu J
FAU - Yu, Jun-Tang
AU  - Yu JT
FAU - Zhang, Yong-Lian
AU  - Zhang YL
LA  - eng
PT  - Journal Article
PL  - China
TA  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
JT  - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
JID - 20730160R
EDAT- 2002/08/09 10:00
MHDA- 2002/08/09 10:00
CRDT- 2002/08/09 10:00
PHST- 2002/08/09 10:00 [pubmed]
PHST- 2002/08/09 10:00 [medline]
PHST- 2002/08/09 10:00 [entrez]
PST - ppublish
SO  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(6):618-622.