PMID- 12168108
OWN - NLM
STAT- MEDLINE
DCOM- 20030324
LR  - 20041117
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 21
IP  - 3
DP  - 2002 Sep
TI  - Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.
PG  - 617-20
AB  - Dendritic cells (DCs) are known to be generated from leukemic clone from patients 
      with acute and chronic leukemia when cultured in the presence of combination of 
      granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis 
      factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports 
      that showed DCs could be effectively differentiated from human leukemia cell 
      lines. In this study, we have shown that a human monoblastic cell line, UG3, was 
      inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along 
      monocyte-macrophage lineage. These DCs, consistently displayed dendritic 
      morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus 
      may represent a suitable model to further elucidate characteristics of DC 
      differentiation.
FAU - Fujii, Nobuharu
AU  - Fujii N
AD  - Second Department of Internal Medicine, Okayama University Medical School, 2-5-1 
      Shikata-cho, Okayama 700-8558, Japan.
FAU - Ikeda, Takashi
AU  - Ikeda T
FAU - Ikeda, Kazuma
AU  - Ikeda K
FAU - Hiraki, Akio
AU  - Hiraki A
FAU - Kawakami, Kimihiko
AU  - Kawakami K
FAU - Masuda, Kozo
AU  - Masuda K
FAU - Maeda, Yoshinobu
AU  - Maeda Y
FAU - Hatake, Kiyohiko
AU  - Hatake K
FAU - Motoyoshi, Kazuo
AU  - Motoyoshi K
FAU - Harada, Mine
AU  - Harada M
FAU - Tanimoto, Mitsune
AU  - Tanimoto M
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Line
MH  - Dendritic Cells/*cytology/drug effects/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocyte Activation/immunology
MH  - Monocytes/*cytology/drug effects/immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2002/08/09 10:00
MHDA- 2003/03/26 04:00
CRDT- 2002/08/09 10:00
PHST- 2002/08/09 10:00 [pubmed]
PHST- 2003/03/26 04:00 [medline]
PHST- 2002/08/09 10:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2002 Sep;21(3):617-20.