PMID- 12168732
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20220408
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 19
IP  - 7
DP  - 2002 Jul
TI  - Sperm single-stranded DNA, detected by acridine orange staining, reduces 
      fertilization and quality of ICSI-derived embryos.
PG  - 319-28
AB  - PURPOSE: The aim of this study was to evaluate the effect of sperm 
      single-stranded DNA, detected by acridine orange (AO), and classical sperm 
      parameters on embryonic quality after ICSI. METHODS: Before ICSI, the spermatozoa 
      of 183 infertile patients with oligo-, astheno-, teratozoospermia (n = 147), or 
      more than one previous unsuccessful conventional IVF attempt (n = 36) were 
      stained by AO to assess the presence of single-stranded DNA. Two days after ICSI, 
      the embryos of 135 patients were scored for morphology, fragmentation included. 
      Embryos of 48 couples were cultured for 4 days to develop to the morula or 
      blastocyst stage. At most 2 embryos were transferred on Day 2 or 4. RESULTS: When 
      the level of spermatozoa with single-stranded DNA was increased, there was a 
      significantly lower fertilization rate after ICSI. Besides, increased sperm 
      single-stranded DNA resulted in a higher proportion of heavily fragmented embryos 
      on Day 2 (P < 0.05). In patients with an increased level of spermatozoa with 
      single-stranded DNA, a significantly higher number of embryos were arrested in 
      spite of prolonged culturing (P < 0.05). Classical sperm parameters did not 
      affect the quality and developmental potential of ICSI-derived embryos. No 
      correlation was found between the level of spermatozoa with single-stranded DNA, 
      pregnancy rate, and live-birth rate achieved by ICSI, except in patients with 0% 
      of spermatozoa with single-stranded DNA, in whom the pregnancy rate was 
      significantly higher. CONCLUSIONS: Sperm single-stranded DNA provides additional 
      data on sperm functional capacity in terms of fertilization and embryonic quality 
      after ICSI.
FAU - Virant-Klun, Irma
AU  - Virant-Klun I
AD  - Department of Obstetrics and Gynecology, Medical Centre Ljubljana, Slovenia. 
      irma.virant@kclj.si
FAU - Tomazevic, Tomaz
AU  - Tomazevic T
FAU - Meden-Vrtovec, Helena
AU  - Meden-Vrtovec H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Adult
MH  - DNA, Single-Stranded/*physiology
MH  - Embryo Transfer
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - Male
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Sperm Capacitation/*genetics
MH  - *Sperm Injections, Intracytoplasmic
MH  - Spermatozoa/metabolism/*physiology
PMC - PMC3455751
EDAT- 2002/08/10 10:00
MHDA- 2003/02/07 04:00
PMCR- 2003/07/01
CRDT- 2002/08/10 10:00
PHST- 2002/08/10 10:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2002/08/10 10:00 [entrez]
PHST- 2003/07/01 00:00 [pmc-release]
AID - 373893 [pii]
AID - 10.1023/a:1016006509036 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2002 Jul;19(7):319-28. doi: 10.1023/a:1016006509036.