PMID- 12168847
OWN - NLM
STAT- MEDLINE
DCOM- 20020913
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 22
IP  - 3
DP  - 2002 May-Jun
TI  - Tumor necrosis factor-alpha induces apoptosis associated with poly(ADP-ribose) 
      polymerase cleavage in HT-29 colon cancer cells.
PG  - 1635-9
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is known for its selective 
      cytotoxic activity on tumour cells. We analysed the response of HT-29 human colon 
      carcinoma cells to this cytokine. MATERIALS AND METHODS: After TNF-alpha 
      treatment, cell proliferation, cell cycle, reactive oxygen species (ROS) 
      production (flow cytometry), the amount of apoptotic cells (flow cytometry, 
      fluorescence microscopy), cleavage of poly (ADP-ribose) polymerase (PARP) and 
      caspase-3 activity (Western blotting) were detected. RESULTS: TNF-alpha induced a 
      decrease of cell growth and viability, an accumulation of cells in the S-phase of 
      the cell cycle, an increase of subdiploid cell population and nuclear chromatin 
      condensation and fragmentation, but not sooner than 96-120 hours. However, 
      earlier events characteristic of apoptosis occurred, such as caspase-3 
      activation, PARP cleavage to 89 kDa fragment and changes in ROS production. 
      CONCLUSION: We demonstrated that, in addition to being an early marker of 
      apoptosis, activation of caspase-3 and degradation of PARP may play a causative 
      role in HT-29 cell death induced by TNF-alpha.
FAU - Vaculova, Alena
AU  - Vaculova A
AD  - Laboratory of Cytokinetics, Institute of Biophysics, Academy of Sciences, Brno, 
      Czech Republic.
FAU - Hofmanova, Jirina
AU  - Hofmanova J
FAU - Soucek, Karel
AU  - Soucek K
FAU - Kovarikova, Martina
AU  - Kovarikova M
FAU - Kozubik, Alois
AU  - Kozubik A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Cell Death/drug effects
MH  - Cell Division/drug effects
MH  - HT29 Cells/*drug effects/enzymology/pathology
MH  - Humans
MH  - Kinetics
MH  - Poly(ADP-ribose) Polymerases/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2002/08/10 10:00
MHDA- 2002/09/14 10:01
CRDT- 2002/08/10 10:00
PHST- 2002/08/10 10:00 [pubmed]
PHST- 2002/09/14 10:01 [medline]
PHST- 2002/08/10 10:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2002 May-Jun;22(3):1635-9.