PMID- 12169273
OWN - NLM
STAT- MEDLINE
DCOM- 20020913
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 278
IP  - 2
DP  - 2002 Aug 15
TI  - Menin's interaction with glial fibrillary acidic protein and vimentin suggests a 
      role for the intermediate filament network in regulating menin activity.
PG  - 175-83
AB  - Recently the multiple endocrine neoplasia type 1 (MEN1) tumor suppressor gene was 
      cloned. Its protein product, called menin, has been shown to associate with the 
      AP1 transcription factor JunD and to repress JunD-mediated transcription. 
      However, little is known concerning the regulation of menin. Here we report that 
      menin interacts with the type III intermediate filament (IF) proteins glial 
      fibrillary acidic protein (GFAP) and vimentin. Menin's interaction with these IF 
      proteins was characterized and confirmed both in vitro and in vivo using GST 
      pull-down analysis, co-immunoprecipitation experiments, and immunofluorescence 
      studies. Deletion mutants of GFAP or vimentin involving the head domains of the 
      molecules abolish the interaction with menin. Endogenous menin is colocalized 
      with GFAP and vimentin in glioma cells as determined by confocal microscopy. 
      Furthermore, a tailless GFAP deletion mutant, which disrupts the IF network, 
      results in menin/GFAP/vimentin-containing aggregates. Triple immunofluorescence 
      labeling studies with antibodies against menin, BrdU, and GFAP show that menin 
      and GFAP colocalize in glioma cells at the S-G2 phase of the cell cycle, as 
      measured by BrdU incorporation. Our data suggest that the intermediate filament 
      network interacts with and may serve as a cytoplasmic sequestering network for 
      menin at the S and early G2 phase of the cell cycle.
FAU - Lopez-Egido, Juan
AU  - Lopez-Egido J
AD  - Department of Medical Sciences, Endocrine Oncology Unit, Uppsala University 
      Hospital, S-751 85 Uppsala, Sweden.
FAU - Cunningham, Janet
AU  - Cunningham J
FAU - Berg, Mikael
AU  - Berg M
FAU - Oberg, Kjell
AU  - Oberg K
FAU - Bongcam-Rudloff, Erik
AU  - Bongcam-Rudloff E
FAU - Gobl, Anders
AU  - Gobl A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Vimentin)
SB  - IM
MH  - Gene Library
MH  - Glial Fibrillary Acidic Protein/genetics/*metabolism
MH  - Humans
MH  - Intermediate Filaments/*physiology
MH  - Interphase
MH  - Microscopy, Fluorescence
MH  - Mutation
MH  - Neoplasm Proteins/*metabolism
MH  - Protein Binding
MH  - *Proto-Oncogene Proteins
MH  - Tumor Cells, Cultured
MH  - Two-Hybrid System Techniques
MH  - Vimentin/genetics/*metabolism
EDAT- 2002/08/10 10:00
MHDA- 2002/09/14 10:01
CRDT- 2002/08/10 10:00
PHST- 2002/08/10 10:00 [pubmed]
PHST- 2002/09/14 10:01 [medline]
PHST- 2002/08/10 10:00 [entrez]
AID - S0014482702955758 [pii]
AID - 10.1006/excr.2002.5575 [doi]
PST - ppublish
SO  - Exp Cell Res. 2002 Aug 15;278(2):175-83. doi: 10.1006/excr.2002.5575.