PMID- 12169446
OWN - NLM
STAT- MEDLINE
DCOM- 20020923
LR  - 20161124
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 283
IP  - 3
DP  - 2002 Sep
TI  - Enhancement of the inducible NO synthase activation by retinoic acid is mimicked 
      by RARalpha agonist in vivo.
PG  - E525-35
AB  - We have previously shown that all-trans retinoic acid (atRA), the active 
      metabolite of vitamin A, enhances the activation of the inducible nitric oxide 
      synthase (NOS II) pathway, a component of innate immunity, in rats in vivo. We 
      investigated the relative contribution of retinoic acid receptor-alpha (RARalpha) 
      and retinoid X receptors (RXRs) to NOS II activation triggered by LPS. Five-day 
      supplementation with 10 mg/kg of either atRA or the RARalpha selective agonist 
      Ro-40-6055, but not with 10 mg/kg of the pan-RXR agonist Ro-25-7386, enhanced the 
      LPS-induced NOS II mRNA, protein expression in liver, and plasma nitrite/nitrate 
      concentration. Both atRA and the RARalpha agonist (but not the RXR agonist) 
      increased the number of peripheral T helper lymphocytes and plasma 
      interferon-gamma concentration. Synergism between retinoids and LPS on NOS II 
      activation within an organ coincided with synergism on interferon regulatory 
      factor-1 mRNA expression but not with the level of expression of the RARalpha 
      protein. These results suggest that, in vivo, atRA activates NOS II through 
      RARalpha and contributes to characterizing the complex effect of retinoids on the 
      host inflammatory/immune response.
FAU - Seguin-Devaux, Carole
AU  - Seguin-Devaux C
AD  - Unite Propre de Recherche et d'Enseignement Superieur-Equipe d'Accueil 3447 
      Lesions-Reparation: Remodelage Cardiaque et Arteriel, Faculte de Medecine, 
      Universite Henri Poincare, Nancy I, France.
FAU - Devaux, Yvan
AU  - Devaux Y
FAU - Latger-Cannard, Veronique
AU  - Latger-Cannard V
FAU - Grosjean, Sandrine
AU  - Grosjean S
FAU - Rochette-Egly, Cecile
AU  - Rochette-Egly C
FAU - Zannad, Faiez
AU  - Zannad F
FAU - Meistelman, Claude
AU  - Meistelman C
FAU - Mertes, Paul-Michel
AU  - Mertes PM
FAU - Longrois, Dan
AU  - Longrois D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Benzoates)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interferon Regulatory Factor-1)
RN  - 0 (Irf1 protein, rat)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rara protein, rat)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoic Acid Receptor alpha)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Tetrahydronaphthalenes)
RN  - 0 (Transcription Factors)
RN  - 102121-60-8 (Am 580)
RN  - 5688UTC01R (Tretinoin)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
SB  - IM
MH  - Animals
MH  - Benzoates/*pharmacology
MH  - Blood Cell Count
MH  - DNA-Binding Proteins/genetics
MH  - Enzyme Activation/drug effects
MH  - Interferon Regulatory Factor-1
MH  - Interferon-gamma/blood/genetics
MH  - Liver/drug effects/metabolism
MH  - Lung/drug effects/metabolism
MH  - Male
MH  - Nitric Oxide Synthase/genetics/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Phosphoproteins/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred WKY
MH  - Receptors, Retinoic Acid/*agonists/metabolism
MH  - Retinoic Acid Receptor alpha
MH  - Retinoid X Receptors
MH  - Spleen/drug effects/metabolism
MH  - T-Lymphocyte Subsets/cytology/drug effects
MH  - Tetrahydronaphthalenes/*pharmacology
MH  - Transcription Factors/agonists
MH  - Tretinoin/*pharmacology
EDAT- 2002/08/10 10:00
MHDA- 2002/09/24 06:00
CRDT- 2002/08/10 10:00
PHST- 2002/08/10 10:00 [pubmed]
PHST- 2002/09/24 06:00 [medline]
PHST- 2002/08/10 10:00 [entrez]
AID - 10.1152/ajpendo.00008.2002 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2002 Sep;283(3):E525-35. doi: 
      10.1152/ajpendo.00008.2002.