PMID- 12169675
OWN - NLM
STAT- MEDLINE
DCOM- 20030122
LR  - 20181113
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 4
IP  - 3
DP  - 2002 Aug
TI  - Rapid detection of IgH/BCL2 rearrangement in follicular lymphoma by interphase 
      fluorescence in situ hybridization with bacterial artificial chromosome probes.
PG  - 144-9
AB  - Follicular lymphomas (FLs) can be difficult to diagnose on aspirated specimens 
      since the architectural pattern is not present. FLs characteristically have 
      rearrangements in the IgH and BCL2 genes resulting from the reciprocal t(14;18) 
      (q32; q21) translocation. Because of the dispersed distribution of breakpoints, 
      fluorescence in situ hybridization (FISH) using genomic probes that span or flank 
      the breakpoints is ideal for detecting this rearrangement in fine-needle 
      aspiration (FNA) biopsies. To develop a set of probes, a bacterial artificial 
      chromosome library was screened and the clones were mapped by fiber FISH. The 
      probes were produced by the direct incorporation of fluorochrome-labeled 
      nucleotides. The colocalization base FISH assay was applied to Cytospin 
      preparations from FNA biopsies of lymph nodes from 26 patients with FL and 10 
      patients without FL. In those with FL, the percentage of cells with at least one 
      IgH/BCL2 fusion signal ranged from 22% to 100% (mean, 63%), which was 
      statistically significantly higher than that in FL-negative samples (mean, 2.7%). 
      The probes demonstrated a significantly lower cutoff value (7%) in normal 
      controls and effectively reduced the false-positive rate in FL-negative cases. 
      These results were confirmed with fiber FISH assays on the same specimens. This 
      interphase FISH assay is rapid and reliable for detecting rearrangements in the 
      IGH/BCL2 gene, thereby aiding in the diagnosis of FL on FNA biopsy specimens.
FAU - Jiang, Feng
AU  - Jiang F
AD  - Department of Pathology, The University of Texas M. D. Anderson Cancer Center, 
      Houston, Texas, USA.
FAU - Lin, Fan
AU  - Lin F
FAU - Price, Roger
AU  - Price R
FAU - Gu, Jun
AU  - Gu J
FAU - Medeiros, L Jeffrey
AU  - Medeiros LJ
FAU - Zhang, Hua Z
AU  - Zhang HZ
FAU - Xie, Su-Su
AU  - Xie SS
FAU - Caraway, Nancy P
AU  - Caraway NP
FAU - Katz, Ruth L
AU  - Katz RL
LA  - eng
GR  - N01CN85083/CA/NCI NIH HHS/United States
GR  - P50 CA070907/CA/NCI NIH HHS/United States
GR  - N01 CN 85053 57/CN/NCI NIH HHS/United States
GR  - P50 CA70907/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - *Chromosomes, Artificial, Bacterial
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphoma, Follicular/*genetics
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
PMC - PMC1906983
EDAT- 2002/08/10 10:00
MHDA- 2003/01/23 04:00
PMCR- 2003/08/01
CRDT- 2002/08/10 10:00
PHST- 2002/08/10 10:00 [pubmed]
PHST- 2003/01/23 04:00 [medline]
PHST- 2002/08/10 10:00 [entrez]
PHST- 2003/08/01 00:00 [pmc-release]
AID - S1525-1578(10)60695-2 [pii]
AID - 0094 [pii]
AID - 10.1016/S1525-1578(10)60695-2 [doi]
PST - ppublish
SO  - J Mol Diagn. 2002 Aug;4(3):144-9. doi: 10.1016/S1525-1578(10)60695-2.