PMID- 12172622
OWN - NLM
STAT- MEDLINE
DCOM- 20021030
LR  - 20171116
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 59
IP  - 4-5
DP  - 2002 Aug
TI  - Biodegradation of monohalogenated alkanes by soil NH(3)-oxidizing bacteria.
PG  - 535-9
AB  - Although cooxidative biodegradation of monohalogenated hydrocarbons has been well 
      studied in the model NH(3)-oxidizing bacterium, Nitrosomonas europaea, virtually 
      no information exists about cooxidation of these compounds by native populations 
      of NH(3)-oxidizing bacteria. To address this subject, nitrifying activity was 
      stimulated to 125-400 nmol NO(3)(-) produced g(-1) soil h(-1) by first incubating 
      a Ca(OH)(2)-amended, silt loam soil (pH 7.0+/-0.2) at field capacity (270 g H(2)O 
      kg(-1) soil) with 10 micro mol NH(4)(+) g(-1) soil for 14 days, followed by 
      another 10 days of incubation in a shaken slurry (2:1 water:soil, v/w) with 
      periodic pH adjustment and maintenance of 10 mM NH(4)(+). These slurries actively 
      degraded both methyl bromide (MeBr) and ethyl chloride (EtCl) at maximum rates of 
      20-30 nmol ml(-1) h(-1) that could be sustained for approximately 12 h. Although 
      the MeBr degradation rates were linear for the first 10-12 h of incubation, they 
      could not be sustained regardless of NH(4)(+) level and declined to zero over 20 
      h of incubation. The transformation capacity of the slurry enrichments (~1 micro 
      mol MeBr ml(-1) soil slurry) was similar to the value measured previously in cell 
      suspensions of N. europaea with similar NH(3)-oxidizing activity. Several 
      MeBr-degrading characteristics of the nitrifying enrichments were found to be 
      similar to those documented in the literature for MeBr-degrading methanotrophs 
      and facultatively methylotrophic bacteria.
FAU - Duddleston, K N
AU  - Duddleston KN
AD  - Department of Microbiology, Oregon State University, Corvallis, OR 97331-3804, 
      USA.
FAU - Arp, D J
AU  - Arp DJ
FAU - Bottomley, P J
AU  - Bottomley PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20020601
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Alkanes)
RN  - 0 (Culture Media)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Hydrocarbons, Halogenated)
RN  - 0 (Soil)
RN  - 46U771ERWK (Ethyl Chloride)
RN  - 7664-41-7 (Ammonia)
RN  - 9V42E1Z7B6 (methyl bromide)
SB  - IM
MH  - Alkanes/*metabolism
MH  - Ammonia/*metabolism
MH  - Bacteria/*metabolism
MH  - Biodegradation, Environmental
MH  - Culture Media
MH  - Ethyl Chloride/metabolism
MH  - Hydrocarbons, Brominated/metabolism
MH  - Hydrocarbons, Halogenated/*metabolism
MH  - Nitrosomonas/metabolism
MH  - Oxidation-Reduction
MH  - Soil
MH  - *Soil Microbiology
EDAT- 2002/08/13 10:00
MHDA- 2002/11/01 04:00
CRDT- 2002/08/13 10:00
PHST- 2002/02/05 00:00 [received]
PHST- 2002/04/10 00:00 [revised]
PHST- 2002/04/19 00:00 [accepted]
PHST- 2002/08/13 10:00 [pubmed]
PHST- 2002/11/01 04:00 [medline]
PHST- 2002/08/13 10:00 [entrez]
AID - 10.1007/s00253-002-1031-7 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2002 Aug;59(4-5):535-9. doi: 
      10.1007/s00253-002-1031-7. Epub 2002 Jun 1.