PMID- 12173727
OWN - NLM
STAT- MEDLINE
DCOM- 20030129
LR  - 20161124
IS  - 0394-3402 (Print)
IS  - 0394-3402 (Linking)
VI  - 15
IP  - 3
DP  - 2002 Jun
TI  - Insulin effect on leucine kinetics in type 2 diabetes mellitus.
PG  - 136-42
AB  - Insulin-induced glucose disposal is impaired in Type 2 diabetes mellitus (T2DM). 
      To determine whether insulin-induced suppression of protein breakdown also is 
      impaired, we measured leucine flux (an index of protein breakdown) in diabetic 
      and nondiabetic subjects during a hyperinsulinemic euglycemic clamp. To avoid the 
      confounding effects of a difference in baseline glucose, glucose concentration in 
      the diabetic subjects was normalized by means of an overnight insulin infusion. 
      Despite higher plasma insulin levels (33.5+/-0.05 vs 132+/-2.7 pmol/l, p<01) 
      diabetic subjects had similar amino acid concentrations and leucine flux 
      (96.9+/-5.8 vs 93.4+/-3.7 micromol/kg/h) as nondiabetic subjects. Infusion of 
      insulin (0.5 mU/kg/min) increased insulin levels (p<0.01) to identical levels in 
      both groups (218+/-16 vs 222+/-19), but the glucose infusion required to maintain 
      euglycemia was higher (p<0.01) in nondiabetic than in diabetic subjects, 
      indicating insulin resistance to glucose disposal in the diabetic subjects. In 
      contrast, leucine flux (81.3+/-4.8 vs 81.6+/-3.4 micromol/kg/h) reached identical 
      levels in both groups. The individual and total amino acid levels also were 
      comparable in both groups. We conclude that suppression of whole body protein 
      turnover in response to an acute increase in insulin is normal in people with 
      T2DM. However, chronic adaptation to high insulin levels occurs, thereby enabling 
      protein breakdown and amino acid concentration to remain within the normal range 
      in people with T2DM.
FAU - Halvatsiotis, P G
AU  - Halvatsiotis PG
AD  - Division of Endocrinology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
FAU - Turk, D
AU  - Turk D
FAU - Alzaid, A
AU  - Alzaid A
FAU - Dinneen, S
AU  - Dinneen S
FAU - Rizza, R A
AU  - Rizza RA
FAU - Nair, K S
AU  - Nair KS
LA  - eng
GR  - DK41973/DK/NIDDK NIH HHS/United States
GR  - R01 DK 29953/DK/NIDDK NIH HHS/United States
GR  - RR00585/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Italy
TA  - Diabetes Nutr Metab
JT  - Diabetes, nutrition & metabolism
JID - 8813443
RN  - 0 (Blood Glucose)
RN  - 0 (C-Peptide)
RN  - 0 (Caproates)
RN  - 0 (Carbon Isotopes)
RN  - 0 (Insulin)
RN  - 0 (Proteins)
RN  - 4G4U8JA28T (isocaproic acid)
RN  - GMW67QNF9C (Leucine)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Blood Glucose/metabolism
MH  - Body Mass Index
MH  - C-Peptide/blood
MH  - Caproates/blood
MH  - Carbon Isotopes
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Female
MH  - Glucose/administration & dosage
MH  - Glucose Clamp Technique
MH  - Humans
MH  - Insulin/administration & dosage/blood/*pharmacology
MH  - Kinetics
MH  - Leucine/*blood
MH  - Male
MH  - Middle Aged
MH  - Proteins/metabolism
EDAT- 2002/08/14 10:00
MHDA- 2003/01/30 04:00
CRDT- 2002/08/14 10:00
PHST- 2002/08/14 10:00 [pubmed]
PHST- 2003/01/30 04:00 [medline]
PHST- 2002/08/14 10:00 [entrez]
PST - ppublish
SO  - Diabetes Nutr Metab. 2002 Jun;15(3):136-42.