PMID- 12174295 OWN - NLM STAT- Publisher LR - 20191120 IS - 0582-9879 (Print) IS - 0582-9879 (Linking) VI - 30 IP - 1 DP - 1998 TI - Cloning of DNA Fragments Possessing Promoter Function from Kluyveromyces cicerisporus. PG - 35-40 AB - Using three yeast promoter-probe plasmids pSK-kan401, pSK-kan1105, pSK-kan1238 with different reading frames, eight DNA fragments possessing high efficient promoter function have been cloned from K. cicerisporus. Their 3'-DNA sequences have been analyzed. The relative strength of their promoter function was studied by comparing their ability to promote the expression of the reporter gene APHI with that of glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) promoter in K. lactis. The results showed that the insertion fragments in pSK-kan401-41 and pSK-kan1105-51 have stronger promoter function and they are also functional in K. cicerisporus Y179. FAU - Xu, Guo-Zhou AU - Xu GZ AD - State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. FAU - Pan, Feng AU - Pan F FAU - Huo, Ke-Ke AU - Huo KK FAU - Li, Yu-Yang AU - Li YY LA - eng PT - Journal Article PL - China TA - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) JT - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica JID - 20730160R EDAT- 2002/08/14 10:00 MHDA- 2002/08/14 10:00 CRDT- 2002/08/14 10:00 PHST- 2002/08/14 10:00 [pubmed] PHST- 2002/08/14 10:00 [medline] PHST- 2002/08/14 10:00 [entrez] PST - ppublish SO - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(1):35-40.