PMID- 12175727
OWN - NLM
STAT- MEDLINE
DCOM- 20030303
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 63
IP  - 9
DP  - 2002 Sep
TI  - Anti-peptide antibodies that recognize conformational differences of HLA class I 
      intracytoplasmic domains.
PG  - 731-41
AB  - Rabbit antibodies were raised against both long and short peptides derived from 
      exon 7 sequences of human leukocyte antigen (HLA) class I alpha chains; anti-A/B 
      against a 13-mer shared by most HLA-A alpha and HLA-B alpha chains, anti-C 
      against a 15-mer characteristic of HLA-C alpha chains, anti-ACT against a 6-mer 
      specific to HLA-A alpha chains, and anti-CCT against a 5-mer specific to HLA-C 
      alpha chains. Binding activity of the antibodies was determined with peptides by 
      enzyme-linked immunoabsorbent assay (ELISA) and with HLA class I transfectants 
      and the parental cells by FACS analysis. Anti-A/B and anti-C were to a greater or 
      lesser extent crossreactive with the long and short peptides, whereas anti-ACT 
      and anti-CCT were specific to the corresponding short peptides. No binding was 
      seen for anti-ACT and anti-CCT with HLA class I transfectants, C1R-A2, C1R-B7, 
      and 221-Cw1 and the parental cells, C1R (Cw4, E) and 721.221 (E, F). Anti-A/B and 
      anti-C were substantially protein-reactive and the binding order was C1R-B7 > 
      C1R-A2, 721.221 > C1R, 221-Cw1 for anti-A/B, and C1R-B7 > 721.221 > C1R, 221-Cw1, 
      C1R-A2 for anti-C. Thus, anti-A/B and anti-C bound better to HLA-B and HLA-E 
      rather than to HLA-A and HLA-C. Computer modeling of the three-dimensional 
      structure of the intracytoplasmic domains demonstrated that this may be due to 
      structural differences despite the sequence similarities.
FAU - Chersi, Alberto
AU  - Chersi A
AD  - Regina Elena Institute for Cancer Research, Rome, Italy.
FAU - Galati, Rossella
AU  - Galati R
FAU - Ogino, Takeshi
AU  - Ogino T
FAU - Butler, Richard H
AU  - Butler RH
FAU - Tanigaki, Nobuyuki
AU  - Tanigaki N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Protein Subunits)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Antigen-Antibody Reactions
MH  - Cell Line
MH  - Computer Simulation
MH  - Cytoplasm/immunology
MH  - Histocompatibility Antigens Class I/*chemistry/genetics/*immunology
MH  - Humans
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Protein Structure, Tertiary
MH  - Protein Subunits
MH  - Rabbits
MH  - Transfection
EDAT- 2002/08/15 10:00
MHDA- 2003/03/04 04:00
CRDT- 2002/08/15 10:00
PHST- 2002/08/15 10:00 [pubmed]
PHST- 2003/03/04 04:00 [medline]
PHST- 2002/08/15 10:00 [entrez]
AID - S0198885902004287 [pii]
AID - 10.1016/s0198-8859(02)00428-7 [doi]
PST - ppublish
SO  - Hum Immunol. 2002 Sep;63(9):731-41. doi: 10.1016/s0198-8859(02)00428-7.