PMID- 12175855 OWN - NLM STAT- MEDLINE DCOM- 20021217 LR - 20200106 IS - 0736-5748 (Print) IS - 1873-474X (Electronic) IS - 0736-5748 (Linking) VI - 20 IP - 3-5 DP - 2002 Jun-Aug TI - Modulation of neuronal calcium signaling by neurotrophic factors. PG - 199-207 AB - Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin (NT) family, is emerging as a key mediator of activity-dependent modifications of synaptic strength in the central nervous system. Because of the well-established role of post-synaptic elevations in concentrations of free Ca(2+) ions ([Ca(2+)](i)) in synaptic plasticity, we investigated the hypothesis that BDNF exerts its neuromodulatory effects on hippocampal pyramidal neurons by enhancing dendritic [Ca(2+)](i) transients mediated by voltage-dependent Ca(2+) channels (VDCCs) during the firing of back-propagating action potentials. Simultaneous whole-cell recording and microfluorometric Ca(2+) imaging were performed in CA1 pyramidal neurons from hippocampal organotypic slice cultures treated with BDNF for 2-4 days in vitro. Our observations indicate that long-term exposure to BDNF does not affect [Ca(2+)](i) transients in apical dendrites mediated by influx through L-type VDCCs during trains of back-propagating action potentials evoked by direct depolarizing current injections. These results suggest that, despite BDNF's profound effects on hippocampal synaptic plasticity, and of L-type Ca(2+) channels on neuronal gene transcription, the role of BDNF in cellular models of hippocampus-dependent learning and memory does not involve modulation of voltage-gated dendritic Ca(2+) signaling mediated by L-type channels in apical dendrites of CA1 pyramidal neurons. FAU - McCutchen, Mary Eve AU - McCutchen ME AD - Department of Neurobiology, CIRC-429, University of Alabama at Birmingham, Birmingham, AL 35294-0021, USA. FAU - Bramham, Clive R AU - Bramham CR FAU - Pozzo-Miller, Lucas D AU - Pozzo-Miller LD LA - eng GR - P30 HD038985/HD/NICHD NIH HHS/United States GR - R01 NS040593/NS/NINDS NIH HHS/United States GR - R01-NS40593-02/NS/NINDS NIH HHS/United States GR - P30 HD38985-02/HD/NICHD NIH HHS/United States GR - R01 NS040593-09/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Int J Dev Neurosci JT - International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience JID - 8401784 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Calcium Channel Blockers) RN - 0 (Calcium Channels, L-Type) RN - SY7Q814VUP (Calcium) SB - IM MH - Action Potentials/drug effects/physiology MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Calcium/metabolism MH - Calcium Channel Blockers/pharmacology MH - Calcium Channels, L-Type/drug effects/*metabolism MH - Calcium Signaling/drug effects/*physiology MH - Dendrites/drug effects/metabolism MH - Electric Stimulation MH - Gene Expression Regulation/drug effects/physiology MH - Hippocampus/cytology/drug effects/*metabolism MH - Neuronal Plasticity/drug effects/*physiology MH - Pyramidal Cells/cytology/drug effects/*metabolism MH - Rats MH - Synaptic Transmission/drug effects/*physiology PMC - PMC2806852 MID - NIHMS166554 EDAT- 2002/08/15 10:00 MHDA- 2002/12/18 04:00 PMCR- 2010/01/14 CRDT- 2002/08/15 10:00 PHST- 2002/08/15 10:00 [pubmed] PHST- 2002/12/18 04:00 [medline] PHST- 2002/08/15 10:00 [entrez] PHST- 2010/01/14 00:00 [pmc-release] AID - S073657480200014X [pii] AID - 10.1016/s0736-5748(02)00014-x [doi] PST - ppublish SO - Int J Dev Neurosci. 2002 Jun-Aug;20(3-5):199-207. doi: 10.1016/s0736-5748(02)00014-x.