PMID- 12176901
OWN - NLM
STAT- MEDLINE
DCOM- 20020913
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 100
IP  - 5
DP  - 2002 Sep 1
TI  - A high-resolution allelotype of B-cell chronic lymphocytic leukemia (B-CLL).
PG  - 1787-94
AB  - The most frequent chromosomal aberrations in B-cell chronic lymphocytic leukemia 
      (B-CLL) are deletions on 13q, 11q, and 17p, and trisomy 12, all of which are of 
      prognostic significance. Conventional cytogenetic analysis and fluorescence in 
      situ hybridization (FISH) are used for their detection, but cytogenetic analysis 
      is hampered by the low mitotic index of B-CLL cells, and FISH depends on accurate 
      information about candidate regions. We used a set of 400 highly informative 
      microsatellite markers covering all chromosomal arms (allelotyping) and automated 
      polymerase chain reaction (PCR) protocols to screen 46 patients with typical 
      B-CLL for chromosomal aberrations. For validation, we compared data with our 
      conventional karyotype results and fine mapping with conventional single-site 
      PCR. All clonal cytogenetic abnormalities potentially detectable by our 
      microsatellite PCR (eg, del13q14 and trisomy 12) were picked up. Allelotyping 
      revealed additional complex aberrations in patients with both normal and abnormal 
      B-CLL karyotypes. Aberrations detectable in the samples with our microsatellite 
      panel were found on almost all chromosomal arms. We detected new aberrant loci in 
      typical B-CLL, such as allelic losses on 1q, 9q, and 22q in up to 25% of our 
      patients, and allelic imbalances mirroring chromosomal duplications, 
      amplifications, or aneuploidies on 2q, 10p, and 22q in up to 27% of our patients. 
      We conclude that allelotyping with our battery of informative microsatellites is 
      suitable for molecular screening of B-CLL. The technique is well suited for 
      analyses in clinical trials, it provides a comprehensive view of genetic 
      alterations, and it may identify new loci with candidate genes relevant in the 
      molecular biology of B-CLL.
FAU - Novak, Urban
AU  - Novak U
AD  - Department of Clinical Research and Medical Oncology/Hematology, Inselspital 
      (University Hospital), Bern, Switzerland.
FAU - Oppliger Leibundgut, Elisabeth
AU  - Oppliger Leibundgut E
FAU - Hager, Jorg
AU  - Hager J
FAU - Muhlematter, Dominique
AU  - Muhlematter D
FAU - Jotterand, Martine
AU  - Jotterand M
FAU - Besse, Celine
AU  - Besse C
FAU - Leupin, Nicolas
AU  - Leupin N
FAU - Ratschiller, Daniel
AU  - Ratschiller D
FAU - Papp, Jeanette
AU  - Papp J
FAU - Kearsey, Gina
AU  - Kearsey G
FAU - Aebi, Stefan
AU  - Aebi S
FAU - Graber, Hans
AU  - Graber H
FAU - Jaggi, Rolf
AU  - Jaggi R
FAU - Luthi, Jean-Marc
AU  - Luthi JM
FAU - Meyer-Monard, Sandrine
AU  - Meyer-Monard S
FAU - Lathrop, Mark
AU  - Lathrop M
FAU - Tobler, Andreas
AU  - Tobler A
FAU - Fey, Martin F
AU  - Fey MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - *Alleles
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Humans
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Microsatellite Repeats
MH  - Polymerase Chain Reaction
EDAT- 2002/08/15 10:00
MHDA- 2002/09/14 10:01
CRDT- 2002/08/15 10:00
PHST- 2002/08/15 10:00 [pubmed]
PHST- 2002/09/14 10:01 [medline]
PHST- 2002/08/15 10:00 [entrez]
AID - S0006-4971(20)59265-3 [pii]
PST - ppublish
SO  - Blood. 2002 Sep 1;100(5):1787-94.