PMID- 12182079
OWN - NLM
STAT- MEDLINE
DCOM- 20020829
LR  - 20151119
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 29
IP  - 3
DP  - 2002
TI  - [Physical mapping of the 45S rDNA and 5S rDNA to rice prometaphase chromosome].
PG  - 241-4
AB  - Rice (Oryza sativa L.) is one of the most important cereal crops in the world. 
      The chromosomes of rice are relatively small in size. With the help of 
      fluorescence in situ hybridization (FISH), several rice DNAs have been localized 
      on rice chromosomes. 45S rDNA and 5S rDNA are encoding sequences for ribosomal 
      RNA synthesis. For detecting the chromosomes related to 45S rDNA and 5S rDNA, the 
      digoxigenin-dUTP labeled probe DNA was probed to the prometaphase chromosomes 
      which were prepared from the root tips harvested from an indica rice variety, 
      Zhongxian 3037. For identification of the chromosomes, slides were stained with 
      Giemsa before FISH. With the improved FISH protocol 45S rDNA was clearly detected 
      on two pairs of chromosomes which are usually found to be attached to nucleolus. 
      The signal size between two pairs of the chromosomes was different. According to 
      the characteristics of the chromosomes, the chromosomes with bigger signal were 
      chromosome 9 and the other pair were chromosome 10. They were also discriminated 
      from arm ratio. The signals of 5S rDNA were relatively small but clear. According 
      to the size and arm ratio of the chromosome with FISH signals, the 5S rDNA was 
      mapped on the short arm of chromosome 11, very close to the centromere region.
FAU - Gong, Zhi-Yun
AU  - Gong ZY
AD  - Agriculture College, Yangzhou University, Yangzhou 225009, China. 
      ricegb@yzu.edu.cn
FAU - Wu, Hsin-Kan
AU  - Wu HK
FAU - Cheng, Zhu-Kuan
AU  - Cheng ZK
FAU - Gu, Ming-Hong
AU  - Gu MH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 45S)
SB  - IM
MH  - DNA, Ribosomal/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Oryza/*genetics
MH  - *Physical Chromosome Mapping
MH  - RNA, Ribosomal/*genetics
MH  - RNA, Ribosomal, 5S/*genetics
EDAT- 2002/08/17 10:00
MHDA- 2002/08/30 10:01
CRDT- 2002/08/17 10:00
PHST- 2002/08/17 10:00 [pubmed]
PHST- 2002/08/30 10:01 [medline]
PHST- 2002/08/17 10:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2002;29(3):241-4.