PMID- 12182461
OWN - NLM
STAT- MEDLINE
DCOM- 20030312
LR  - 20191106
IS  - 0966-3274 (Print)
IS  - 0966-3274 (Linking)
VI  - 10
IP  - 1
DP  - 2002 Jun
TI  - Allogeneic chimerism established with a mixture of low dose bone marrow cells and 
      splenocytes in sublethally irradiated mice.
PG  - 25-30
AB  - BACKGROUND: Allogeneic chimerism has been established in graft-accepting 
      recipients and the donor cells in the host may act in a major way to facilitate 
      the induction of tolerance. In this study, we examined the effects of allogeneic 
      chimerism after injecting donor bone marrow cells (BMCs) mixed with splenocytes 
      (SPLCs) to the sublethally conditioned recipients. METHODS: In BALB/c(H-2(d)) to 
      B6(H-2(b)) combination, B6 recipients were irradiated at 7.5 Gy and were injected 
      a mixture of donor BMCs and SPLCs intravenously. On day 90 after injection, the 
      degree of chimerism in peripheral blood lymphocytes (PBL) and in the splenocytes 
      was checked by flow cytometry. RESULTS: In groups which were injected varying 
      BMCs, when > 45 x 10(6) BMCs were injected into B6, a large percentage of donor 
      cells were detected in PBL and in the spleen. In contrast, when < 30 x 10(6) BMCs 
      were injected into B6, only a small percentage of donor cells were detected. In 
      the groups which were injected 3 x 10(6) BMCs with varying SPLCs, when > 10 x 
      10(6) SPLCs were added, a large percentage of donor cells were detected in PBL 
      and SPLCs, but a small percentage of donor cells were detected with the addition 
      of < 3 x 10(6) SPLCs. A high percentage of chimeric mice showed donor specific 
      tolerance in vitro, mixed lymphocyte responses, and in vivo, skin grafting. In 
      contrast, only a small percentage of chimeric mice showed no donor specific 
      tolerance by skin grafting. CONCLUSION: These findings suggested that even a low 
      dose of BMCs can establish a state of allogeneic chimerism and donor specific 
      tolerance if combined with SPLCs.
FAU - Itabashi, Yukihiro
AU  - Itabashi Y
AD  - Second Department of Surgery, Hirosaki University School of Medicine, Japan. 
      itabashi@cc.hirosaki-u.ac.jp
FAU - Narumi, Shunji
AU  - Narumi S
FAU - Hakamada, Kenichi
AU  - Hakamada K
FAU - Watanabe, Nobukazu
AU  - Watanabe N
FAU - Aoki, Kazunori
AU  - Aoki K
FAU - Sasaki, Mutsuo
AU  - Sasaki M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Female
MH  - Graft Survival
MH  - Graft vs Host Disease/prevention & control
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Skin Transplantation/immunology
MH  - Spleen/*cytology
MH  - Transplantation Chimera/*immunology
MH  - Transplantation, Homologous
MH  - Whole-Body Irradiation
EDAT- 2002/08/17 10:00
MHDA- 2003/03/13 04:00
CRDT- 2002/08/17 10:00
PHST- 2002/08/17 10:00 [pubmed]
PHST- 2003/03/13 04:00 [medline]
PHST- 2002/08/17 10:00 [entrez]
AID - S0966-3274(01)00046-6 [pii]
AID - 10.1016/s0966-3274(01)00046-6 [doi]
PST - ppublish
SO  - Transpl Immunol. 2002 Jun;10(1):25-30. doi: 10.1016/s0966-3274(01)00046-6.