PMID- 12182463
OWN - NLM
STAT- MEDLINE
DCOM- 20030312
LR  - 20191106
IS  - 0966-3274 (Print)
IS  - 0966-3274 (Linking)
VI  - 10
IP  - 1
DP  - 2002 Jun
TI  - Efficacy of donor splenocytes mixed with bone marrow cells for induction of 
      tolerance in sublethally irradiated mice.
PG  - 37-41
AB  - BACKGROUND: High dose of bone marrow cells (BMCs) has been reported to be 
      essential to establish donor-specific tolerance. In clinical settings, a large 
      quantity of BMCs is very difficult to be obtained. Our previous report 
      demonstrated that even a low dose of BMCs could establish donor-specific 
      tolerance if mixed with splenocytes (SPLCs). In the present study, various 
      components of SPLCs were purified or removed and were investigated their 
      contribution for enhancement of bone marrow engraftment leading to donor-specific 
      tolerance in sublethally irradiated mice. METHODS: Sublethally irradiated C57BL/6 
      recipient mice were intravenously injected 3 x 10(6) BMCs mixed with various 
      components and various numbers of SPLCs harvested from BALB/c donor mice. One 
      week after injection, skin grafting was performed. The degree of chimerism in 
      peripheral blood lymphocytes (PBLs) and in SPLCs was analyzed by FACS 3 months 
      after transplantation. RESULTS: Recipients receiving 3 X 106 BMCs mixed with 10 x 
      10(6) T cell-enriched SPLCs established chimerism. Recipients receiving BMCs 
      mixed with macrophage-depleted SPLCs also showed chimeirism and donor-specific 
      tolerance. B cell-enriched SPLCs did not help small dose of BMCs to establish 
      chimerism. Irradiated SPLCs were not effective to induce tolerance even with 
      additional infusion to recipients. CONCLUSIONS: Active effects of splenic T cells 
      were more important to help engraftment of small dose of BMCs than B cells, but 
      the interaction between T and B cells might play some roles to enhance BMC 
      engraftment. Splenic macrophages or dendritic cells might have some adverse 
      effects against tolerance induction. Fatal graft-versus-host disease (GVHD) might 
      be avoided by depleting adherent cells from SPLCs, so macrophages or dendritic 
      cells were also considered as key components to induce donor-specific tolerance 
      and prevent GVHD in this model.
FAU - Hashimoto, Naoki
AU  - Hashimoto N
AD  - Second Department of Surgery, Hirosaki University School of Medicine, Japan. 
      hashimo2@cc.hirosaki-u.ac.jp
FAU - Narumi, Shunji
AU  - Narumi S
FAU - Itabashi, Yukihiro
AU  - Itabashi Y
FAU - Hakamada, Kenichi
AU  - Hakamada K
FAU - Sasaki, Mutsuo
AU  - Sasaki M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/physiology
MH  - *Bone Marrow Transplantation
MH  - Graft vs Host Disease/prevention & control
MH  - *Immune Tolerance
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Skin Transplantation/immunology
MH  - Spleen/*cytology
MH  - Transplantation Chimera
MH  - Whole-Body Irradiation
EDAT- 2002/08/17 10:00
MHDA- 2003/03/13 04:00
CRDT- 2002/08/17 10:00
PHST- 2002/08/17 10:00 [pubmed]
PHST- 2003/03/13 04:00 [medline]
PHST- 2002/08/17 10:00 [entrez]
AID - S0966-3274(02)00020-5 [pii]
AID - 10.1016/s0966-3274(02)00020-5 [doi]
PST - ppublish
SO  - Transpl Immunol. 2002 Jun;10(1):37-41. doi: 10.1016/s0966-3274(02)00020-5.