PMID- 12182839
OWN - NLM
STAT- MEDLINE
DCOM- 20030304
LR  - 20191106
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 19
IP  - 2
DP  - 2002 Jul 21
TI  - Human cerebral endothelial cells are a potential source for bioactive BDNF.
PG  - 55-8
AB  - Inflammatory stimuli within the central nervous system may not only induce tissue 
      damage but may also convey neuroprotection. It has been shown that brain derived 
      neurotrophic factor (BDNF) is a neuroprotective candidate. Here we show that BDNF 
      is constitutively expressed in cultured human cerebral endothelial cells (HCEC) 
      and can further be upregulated under proinflammatory conditions. TNF-alpha 
      treatment resulted in an increase in BDNF mRNA expression and protein levels were 
      significantly elevated after 72 h (69+/-33%, P<0.01). Using functional assays it 
      was demonstrated that BDNF produced by HCEC is bioactive and supports motoneuron 
      survival. In contrast, BDNF expression was reduced by TNF-alpha in human 
      umbilical vein endothelial cells (HUVEC). We conclude that HCEC likely to 
      contribute to neuronal survival under physiological and inflammatory conditions.
FAU - Bayas, A
AU  - Bayas A
AD  - Clinical Research Unit for Multiple Sclerosis and Neuroimmunology, Department of 
      Neurology, University of Wurzburg, Germany. antonios.bayas@mail.uni-wuerzburg.de
FAU - Hummel, V
AU  - Hummel V
FAU - Kallmann, B A
AU  - Kallmann BA
FAU - Karch, C
AU  - Karch C
FAU - Toyka, K V
AU  - Toyka KV
FAU - Rieckmann, P
AU  - Rieckmann P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytokines)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/*metabolism/pharmacology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Endothelium/*metabolism
MH  - Humans
MH  - Neurons/drug effects/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Telencephalon/cytology/*metabolism
RF  - 15
EDAT- 2002/08/17 10:00
MHDA- 2003/03/05 04:00
CRDT- 2002/08/17 10:00
PHST- 2002/08/17 10:00 [pubmed]
PHST- 2003/03/05 04:00 [medline]
PHST- 2002/08/17 10:00 [entrez]
AID - S1043466602908926 [pii]
AID - 10.1006/cyto.2002.0892 [doi]
PST - ppublish
SO  - Cytokine. 2002 Jul 21;19(2):55-8. doi: 10.1006/cyto.2002.0892.