PMID- 12184921
OWN - NLM
STAT- MEDLINE
DCOM- 20030211
LR  - 20181130
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 22
IP  - 7
DP  - 2002 Jul
TI  - Pre-pro-B cell growth-stimulating factor (PPBSF) upregulates IL-7Ralpha chain 
      expression and enables pro-B cells to respond to monomeric IL-7.
PG  - 823-32
AB  - Although pro-B cells are well represented in IL-7 knockout (KO) mice, they 
      express abnormally low concentrations of the interleukin-7 receptor alpha-chain 
      (IL-7Ralpha) and do not generate pre-B cells. Here, we demonstrate that pro-B 
      cells from IL-7 KO mice can be induced to generate pre-B cells and immature B 
      cells by exposure to recombinant IL-7 (rIL-7) in vivo but not in vitro. 
      Experiments in recombinant activation gene-1 (RAG-1) KO mice indicate that the in 
      vitro unresponsiveness of IL-7(-/-) pro-B cells to rIL-7 is unrelated to the 
      absence of a functional pre-B cell receptor (pre-BCR). Rather, it appears to be 
      due to the suboptimal expression of the IL-7Ralpha chain. Thus, IL-7(-/-) pro-B 
      cells readily respond to rIL-7 in vitro if IL-7Ralpha chain expression is first 
      upregulated by exposure to IL-7 in vivo or to IL-7(+/+) bone marrow (BM) stromal 
      cells or conditioned medium (CM) therefrom in vitro. Similar results were 
      obtained when pro-B cells from IL-7 KO mice were cultured on IL-7(-/-) BM stromal 
      cells in the presence of rIL-7. This suggested that the recently described 
      pre-pro-B cell growth-stimulating factor (PPBSF), a self-assembling hybrid 
      cytokine comprising IL-7 and the stromal cell-derived hepatocyte growth factor 
      beta-chain (HGFbeta), is required to stimulate pro-B cells from IL-7 KO mice. 
      This inference was verified by demonstrating that purified PPBSF upregulates 
      IL-7Ralpha chain expression on IL-7(-/-) pro-B cells in vitro and enables them to 
      respond to rIL-7 in a stepwise manner. We, therefore, postulate that PPBSF is the 
      operative form of IL-7 that normally induces IL-7Ralpha(lo) pre-pro-B cells to 
      proliferate and differentiate into IL-7Ralpha(hi) pro-B cells, which then 
      proliferate and differentiate into pre-B cells on stimulation with monomeric 
      IL-7.
FAU - Wei, Chiju
AU  - Wei C
AD  - Department of Pathology, School of Medicine, University of Connecticut Health 
      Center, Farmington, CT 06030-3105, USA.
FAU - Lai, Laijun
AU  - Lai L
FAU - Goldschneider, Irving
AU  - Goldschneider I
LA  - eng
GR  - AI32752/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Interleukin-7)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Recombinant Proteins)
RN  - 0 (interleukin-7 receptor, alpha chain)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - B-Lymphocyte Subsets/cytology
MH  - Bone Marrow
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cell Lineage
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/chemistry/*pharmacology
MH  - Culture Media, Conditioned/pharmacology
MH  - Dimerization
MH  - Female
MH  - Genes, RAG-1
MH  - Hepatocyte Growth Factor/chemistry
MH  - Interleukin-7/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Protein Subunits
MH  - Receptors, Interleukin-7/*biosynthesis/genetics
MH  - Recombinant Proteins/pharmacology
MH  - Stromal Cells/cytology/metabolism
MH  - Up-Regulation/drug effects
EDAT- 2002/08/20 10:00
MHDA- 2003/02/13 04:00
CRDT- 2002/08/20 10:00
PHST- 2002/08/20 10:00 [pubmed]
PHST- 2003/02/13 04:00 [medline]
PHST- 2002/08/20 10:00 [entrez]
AID - 10.1089/107999002320271422 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2002 Jul;22(7):823-32. doi: 
      10.1089/107999002320271422.