PMID- 12185311
OWN - NLM
STAT- MEDLINE
DCOM- 20020925
LR  - 20191210
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 64
IP  - 7
DP  - 2002 Jul
TI  - Evaluation of enzyme-linked immunosorbent assay (ELISA) and immunofluorescent 
      antibody (IFA) test for the detection of porcine reproductive and respiratory 
      syndrome virus (PRRSV) antibody in pigs from conventional farms.
PG  - 583-8
AB  - To evaluate the immunofluorescent antibody (IFA) test and enzyme-linked 
      immunosorbent assay (ELISA) for detecting the porcine reproductive and 
      respiratory syndrome virus (PRRSV) antibody, conventional pigs in PRRSV-positive 
      and -negative commercial farms were examined. Antibody development patterns in 
      ELISA and IFA tests were compared in 3 week old piglets experimentally infected 
      with the PRRSV. The virus was detected from 2 days post infection (PI) and then 
      the antibody titers and S/P ratios rose by both methods. A total of 208 serum 
      samples were collected from 4 PRRSV-negative farms and 210 samples from 
      PRRSV-positive farms, and were tested for the PRRSV antibody by IFA and ELISA. 
      The titer of 64 should be set as the cut-off point in IFA for field sera. 
      Similarly, the cut-off S/P ratio should be set at 0.4 in ELISA. A high degree of 
      correlation was observed between antibody titers by the two methods in these 418 
      samples, with a correlation coefficient of 0.84. The coincidence rate between the 
      two tests was 84.7% (354/418). In non-coincident cases, ELISA was able to detect 
      the antibody with a low titer in the serum samples which were negative in IFA but 
      from PRRSV positive farms. ELISA was more sensitive than IFA to detect PRRSV 
      infected animals or farms.
FAU - Yahara, Yoshihiro
AU  - Yahara Y
AD  - Veterinary Service Center, Nisshin Feed Inc., 5-3-1 Tsurugaoka, Oi-machi, 
      Iruma-gun, Saitama 356-8511, Japan.
FAU - Ohkubo, Yukihiro
AU  - Ohkubo Y
FAU - Kariwa, Hiroaki
AU  - Kariwa H
FAU - Takashima, Ikuo
AU  - Takashima I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Antigens, Viral/immunology
MH  - Enzyme-Linked Immunosorbent Assay/*veterinary
MH  - Fluorescent Antibody Technique/*veterinary
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Swine/*immunology/*virology
MH  - Swine Diseases/*diagnosis/immunology/virology
MH  - Weaning
EDAT- 2002/08/20 10:00
MHDA- 2002/09/26 06:00
CRDT- 2002/08/20 10:00
PHST- 2002/08/20 10:00 [pubmed]
PHST- 2002/09/26 06:00 [medline]
PHST- 2002/08/20 10:00 [entrez]
AID - 10.1292/jvms.64.583 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2002 Jul;64(7):583-8. doi: 10.1292/jvms.64.583.