PMID- 12186702
OWN - NLM
STAT- MEDLINE
DCOM- 20030623
LR  - 20221222
IS  - 1024-5332 (Print)
IS  - 1024-5332 (Linking)
VI  - 7
IP  - 2
DP  - 2002 Apr
TI  - MCP-1, MIP-1, IL-8 and ischemic cerebral tissue enhance human bone marrow stromal 
      cell migration in interface culture.
PG  - 113-7
AB  - Bone marrow stromal cells (MSCs) administered intravenously are effective in 
      reducing neurological deficits after stroke in the rodent. These cells appear to 
      selectively migrate and express neural phenotypes in ischemic brain. To elucidate 
      the mechanisms targeting MSC migration into the ischemic brain, we measured, 
      using a microchemotaxis chamber, the effect of select chemotactic factors and 
      cytokines expressed in injured brain, monocyte chemoattractant protein-1 (MCP-1), 
      macrophage inflammatory protein-1alpha (MIP-1alpha) and interleukin-8 (IL-8), on 
      migration of human bone marrow stromal cells (hMSCs). In addition, we 
      investigated whether tissue extracts prepared from rat ischemic brain at various 
      times after middle cerebral artery occlusion (MCAo) induce migration of hMSCs. 
      Our data indicate that MCP-1, MIP-1alpha and IL-8 enhance the migration of hMSCs. 
      Ischemic brain tissue extracts at 24, 48 h and 1 week after ischemia 
      significantly increase hMSC migration across the membrane compared to 
      non-ischemic tissue (p<0.05). These data indicate that hMSCs are targeted by 
      inflammatory chemotactic agents and cytokines and that ischemic brain attracts 
      hMSCs.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202, 
      USA.
FAU - Li, Yi
AU  - Li Y
FAU - Chen, Xiaoguang
AU  - Chen X
FAU - Chen, Jieli
AU  - Chen J
FAU - Gautam, Subhash C
AU  - Gautam SC
FAU - Xu, Yongxian
AU  - Xu Y
FAU - Chopp, Michael
AU  - Chopp M
LA  - eng
GR  - P01NS23393/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Brain Ischemia/metabolism/*pathology
MH  - Chemokine CCL2/pharmacology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokines/*pharmacology
MH  - Chemotaxis/*drug effects
MH  - Coculture Techniques
MH  - Disease Models, Animal
MH  - Humans
MH  - Interleukin-8/pharmacology
MH  - Macrophage Inflammatory Proteins/pharmacology
MH  - Male
MH  - Paracrine Communication
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/*cytology
EDAT- 2002/08/21 10:00
MHDA- 2003/06/24 05:00
CRDT- 2002/08/21 10:00
PHST- 2002/08/21 10:00 [pubmed]
PHST- 2003/06/24 05:00 [medline]
PHST- 2002/08/21 10:00 [entrez]
AID - Y9HJMHKRG6KBT5B5 [pii]
AID - 10.1080/10245330290028588 [doi]
PST - ppublish
SO  - Hematology. 2002 Apr;7(2):113-7. doi: 10.1080/10245330290028588.