PMID- 12186825
OWN - NLM
STAT- MEDLINE
DCOM- 20020906
LR  - 20220331
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 166
IP  - 4
DP  - 2002 Aug 15
TI  - Skeletal muscle apoptosis and weight loss in chronic obstructive pulmonary 
      disease.
PG  - 485-9
AB  - Patients with chronic obstructive pulmonary disease (COPD) often lose weight 
      during the course of their disease. We hypothesized that this may be due to 
      skeletal muscle apoptosis. To investigate this possibility, we obtained 
      quadriceps femoris biopsies in 15 patients with COPD (8 with normal body mass 
      index [BMI] and 7 with low [< 20 kg/m(2)] BMI), 8 healthy volunteers, and 6 
      sedentary subjects undergoing orthopedic surgery (both groups with normal BMI). 
      Skeletal muscle apoptosis was assessed by the transferase-mediated dUTP nick end 
      labeling (TUNEL) technique and the immunodetection of 
      poly-(ADP-ribose)-polymerase proteolytic fragments. Exercise tolerance on a 
      cycloergometer was also determined in patients with COPD. We found that skeletal 
      muscle apoptosis (by both techniques) was increased in patients with COPD and low 
      BMI as compared with the other three groups (p < 0.005). In patients with COPD, 
      BMI was inversely related to skeletal muscle apoptosis (TUNEL, p = 0.009), and it 
      was better correlated with exercise capacity (p = 0.006) than with the degree of 
      airflow obstruction present (p = 0.02). Markers of skeletal muscle apoptosis were 
      not related to any of the measured lung function variables. This study shows that 
      skeletal muscle apoptosis (1) is increased in patients with COPD having low BMI; 
      and (2) is associated with a lower exercise tolerance despite a similar degree of 
      lung function impairment.
FAU - Agusti, Alvar G N
AU  - Agusti AG
AD  - Servei de Pneumologia, Hospital Universitari Son Dureta, Andrea Doria 55, 07014 
      Palma Mallorca, Spain. aagusti@hsd.es
FAU - Sauleda, Jaume
AU  - Sauleda J
FAU - Miralles, Cristina
AU  - Miralles C
FAU - Gomez, Cristina
AU  - Gomez C
FAU - Togores, Bernat
AU  - Togores B
FAU - Sala, Ernest
AU  - Sala E
FAU - Batle, Santiago
AU  - Batle S
FAU - Busquets, Xavier
AU  - Busquets X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
CIN - Am J Respir Crit Care Med. 2002 Aug 15;166(4):434-6. PMID: 12186814
MH  - Aged
MH  - *Apoptosis
MH  - Biopsy
MH  - Blood Gas Analysis
MH  - Blotting, Western
MH  - Body Mass Index
MH  - Case-Control Studies
MH  - Exercise/physiology
MH  - Exercise Tolerance
MH  - Forced Expiratory Volume
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Middle Aged
MH  - Muscle, Skeletal/*pathology
MH  - Muscular Atrophy/*etiology/*pathology
MH  - Poly(ADP-ribose) Polymerases/analysis
MH  - Pulmonary Disease, Chronic Obstructive/*complications/metabolism/*physiopathology
MH  - Severity of Illness Index
MH  - *Weight Loss
EDAT- 2002/08/21 10:00
MHDA- 2002/09/07 10:01
CRDT- 2002/08/21 10:00
PHST- 2002/08/21 10:00 [pubmed]
PHST- 2002/09/07 10:01 [medline]
PHST- 2002/08/21 10:00 [entrez]
AID - 10.1164/rccm.2108013 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2002 Aug 15;166(4):485-9. doi: 10.1164/rccm.2108013.